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Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus
Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH 2-terminal region of phage T7 RNA polymerase as a reporter protein and by s...
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| Published in: | FEBS letters 1996-03, Vol.382 (3), p.330-334 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4669-2126e5cc2e343a01a65f709dfa2be341b02538d7f856b4159aacef8c105f4d643 |
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| cites | cdi_FETCH-LOGICAL-c4669-2126e5cc2e343a01a65f709dfa2be341b02538d7f856b4159aacef8c105f4d643 |
| container_end_page | 334 |
| container_issue | 3 |
| container_start_page | 330 |
| container_title | FEBS letters |
| container_volume | 382 |
| creator | Kügler, S. Grünweller, A. Probst, C. Klinger, M. Müller, P.K. Kruse, C. |
| description | Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH
2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules. |
| doi_str_mv | 10.1016/0014-5793(96)00204-9 |
| format | article |
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2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(96)00204-9</identifier><identifier>PMID: 8605996</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Bacteriophage T7 - enzymology ; Carrier Proteins ; Cell Line ; Cell Nucleus - chemistry ; Chickens ; Cloning, Molecular ; Cytoplasm - chemistry ; DNA-Directed RNA Polymerases - genetics ; Genes, Reporter - genetics ; KH domain ; Liver - chemistry ; Molecular Sequence Data ; Nucleocytoplasmic shuttle ; Protein Structure, Secondary ; Proteins - analysis ; Proteins - chemistry ; Proteins - genetics ; Rats ; Recombinant Fusion Proteins - analysis ; RNA-Binding Proteins ; Subcellular localization ; Vigilin ; Viral Proteins</subject><ispartof>FEBS letters, 1996-03, Vol.382 (3), p.330-334</ispartof><rights>1996</rights><rights>FEBS Letters 382 (1996) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4669-2126e5cc2e343a01a65f709dfa2be341b02538d7f856b4159aacef8c105f4d643</citedby><cites>FETCH-LOGICAL-c4669-2126e5cc2e343a01a65f709dfa2be341b02538d7f856b4159aacef8c105f4d643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579396002049$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3536,27905,27906,45761</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8605996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kügler, S.</creatorcontrib><creatorcontrib>Grünweller, A.</creatorcontrib><creatorcontrib>Probst, C.</creatorcontrib><creatorcontrib>Klinger, M.</creatorcontrib><creatorcontrib>Müller, P.K.</creatorcontrib><creatorcontrib>Kruse, C.</creatorcontrib><title>Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH
2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bacteriophage T7 - enzymology</subject><subject>Carrier Proteins</subject><subject>Cell Line</subject><subject>Cell Nucleus - chemistry</subject><subject>Chickens</subject><subject>Cloning, Molecular</subject><subject>Cytoplasm - chemistry</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Genes, Reporter - genetics</subject><subject>KH domain</subject><subject>Liver - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Nucleocytoplasmic shuttle</subject><subject>Protein Structure, Secondary</subject><subject>Proteins - analysis</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>RNA-Binding Proteins</subject><subject>Subcellular localization</subject><subject>Vigilin</subject><subject>Viral Proteins</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqNkEFP3DAQha2qaLsF_kEr-VTRQ8BOHCe-VAK0C5VW4gJcLceZFFdeO7WTov332LsrjlVP1rz35nn0IfSFkktKKL8ihLKibkR1Ifh3QkrCCvEBLWnbVEXFePsRLd8jn9DnGH-TNLdULNCi5aQWgi_R-Gx-GWsc1t5NyriIFR5mpyfjnbLYzdqCCth6rayJKss4wp8ZnAasXI9NxGOACG7CqaXz0wueXgDr3eRHq-J2H8rKvmqOZ-hkUDbC-fE9RU_r1ePtfbF5uPt5e70pNONcFCUtOdRal1CxShGqeD00RPSDKrsk0Y6UddX2zdDWvGO0FkppGFpNST2wnrPqFH079I7Bp3PjJLcmarBWOfBzlE0jWkpElYLsENTBxxhgkGMwWxV2khKZQctMUWaKUuQhgZYirX099s_dFvr3pSPZ5K8P_quxsPuvTrle3ZTZyLrgezV_9ONQBInWXwNBRm0y_t4E0JPsvfn3pW93hqFv</recordid><startdate>19960318</startdate><enddate>19960318</enddate><creator>Kügler, S.</creator><creator>Grünweller, A.</creator><creator>Probst, C.</creator><creator>Klinger, M.</creator><creator>Müller, P.K.</creator><creator>Kruse, C.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960318</creationdate><title>Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus</title><author>Kügler, S. ; Grünweller, A. ; Probst, C. ; Klinger, M. ; Müller, P.K. ; Kruse, C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4669-2126e5cc2e343a01a65f709dfa2be341b02538d7f856b4159aacef8c105f4d643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bacteriophage T7 - enzymology</topic><topic>Carrier Proteins</topic><topic>Cell Line</topic><topic>Cell Nucleus - chemistry</topic><topic>Chickens</topic><topic>Cloning, Molecular</topic><topic>Cytoplasm - chemistry</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Genes, Reporter - genetics</topic><topic>KH domain</topic><topic>Liver - chemistry</topic><topic>Molecular Sequence Data</topic><topic>Nucleocytoplasmic shuttle</topic><topic>Protein Structure, Secondary</topic><topic>Proteins - analysis</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Rats</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>RNA-Binding Proteins</topic><topic>Subcellular localization</topic><topic>Vigilin</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kügler, S.</creatorcontrib><creatorcontrib>Grünweller, A.</creatorcontrib><creatorcontrib>Probst, C.</creatorcontrib><creatorcontrib>Klinger, M.</creatorcontrib><creatorcontrib>Müller, P.K.</creatorcontrib><creatorcontrib>Kruse, C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kügler, S.</au><au>Grünweller, A.</au><au>Probst, C.</au><au>Klinger, M.</au><au>Müller, P.K.</au><au>Kruse, C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-03-18</date><risdate>1996</risdate><volume>382</volume><issue>3</issue><spage>330</spage><epage>334</epage><pages>330-334</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH
2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8605996</pmid><doi>10.1016/0014-5793(96)00204-9</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1996-03, Vol.382 (3), p.330-334 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77981093 |
| source | Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Bacteriophage T7 - enzymology Carrier Proteins Cell Line Cell Nucleus - chemistry Chickens Cloning, Molecular Cytoplasm - chemistry DNA-Directed RNA Polymerases - genetics Genes, Reporter - genetics KH domain Liver - chemistry Molecular Sequence Data Nucleocytoplasmic shuttle Protein Structure, Secondary Proteins - analysis Proteins - chemistry Proteins - genetics Rats Recombinant Fusion Proteins - analysis RNA-Binding Proteins Subcellular localization Vigilin Viral Proteins |
| title | Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus |
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