Relationship between camp induced inhibition of human meningioma cell proliferation and autocrine secretion of interleukin-6

• Published in: Life sciences (1973) 1996, Vol.58 (16), p.1323-1329
• Main Authors: Hüttner, Anita, Lei, Ting, Fahlbusch, Rudolf, Schrell, Uwe, Adams, Eric F.
• Format: Article
• Language: English
• Subjects: 8-Bromo Cyclic Adenosine Monophosphate - pharmacology; Adult; Aged; Antibodies - pharmacology; autocrine inhibition of growth; Cell Division - drug effects; Cell Division - physiology; cyclic adenosine 3′ 5′ -monophosphate; Cyclic AMP - biosynthesis; Cyclic AMP - pharmacology; Female; Humans; interleukin-6; Interleukin-6 - metabolism; Male; Meningioma - metabolism; Meningioma - pathology; meningiomas; Middle Aged; Neutralization Tests; Theophylline - pharmacology; Tumor Cells, Cultured - drug effects
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/0024-3205(96)00098-7

Previous studies have suggested that activation of the adenylyl cyclase - cAMP system in meningiomas results in decreased mitosis. We have used meningioma cell culture to further investigate this phenomenon and to examine the potential role played by interleukin-6 (IL6). Incubation of cultured meningioma cells for 4–6 days with cholera toxin and theophylline, both of which increase intracellular cAMP levels, markedly stimulated IL6 secretion and inhibited cell growth rate. Similar effects were observed with 8-bromo-cAMP. In contrast, a neutralising polyclonal antibody against IL6 significantly stimulated meningioma proliferation and reduced the inhibitory effects of 8-bromo-cAMP. These results support the concept that IL6 acts as an autocrine / paracrine inhibitory factor for meningioma proliferation, and that the inhibition exerted by elevated intracellular cAMP levels may be at least partially mediated via increased secretion of the cytokine.