Oxidative damage to DNA in diabetes mellitus

Summary Background Increased production of reactive oxygen species (ROS) and lipid peroxidation may contribute to vascular complications in diabetes. To test whether DNA is also oxidatively damaged in diabetes, we measured 8-hydroxydeoxyguanosine (8-OHdG), an indicator of oxidative damage of DNA, in...

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Bibliographic Details
Published in:The Lancet (British edition) 1996-02, Vol.347 (8999), p.444-445
Main Authors: Dandona, P, Thusu, K, Cook, S, Snyder, B, Makowski, J, Armstrong, D, Nicotera, T
Format: Article
Language:English
Subjects:
8-Hydroxydeoxyguanosine
Adult
Aged
Aging
Atherogenesis
Biological and medical sciences
Case-Control Studies
Chemiluminescence
Chromatography, High Pressure Liquid
Damage
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - analysis
Deoxyribonucleic acid
Diabetes
Diabetes mellitus
Diabetes mellitus (insulin dependent)
Diabetes mellitus (non-insulin dependent)
Diabetes Mellitus, Type 1 - metabolism
Diabetes Mellitus, Type 2 - metabolism
Diabetes. Impaired glucose tolerance
Diabetic Angiopathies - etiology
DNA
DNA - chemistry
DNA Damage
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
High performance liquid chromatography
Humans
Insulin
Leukocytes (mononuclear)
Leukocytes, Mononuclear - chemistry
Leukocytes, Mononuclear - metabolism
Lipid Peroxidation
Lipids
Liquid chromatography
Luminescent Measurements
Medical sciences
Middle Aged
Organic chemistry
Oxidation
Patients
Peroxidation
Reactive oxygen species
Reactive Oxygen Species - metabolism
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Summary:Summary Background Increased production of reactive oxygen species (ROS) and lipid peroxidation may contribute to vascular complications in diabetes. To test whether DNA is also oxidatively damaged in diabetes, we measured 8-hydroxydeoxyguanosine (8-OHdG), an indicator of oxidative damage of DNA, in mononuclear cells. Methods For this laboratory-based study, 12 patients with insulin-dependent diabetes mellitus (IDDM) and 15 patients with non-insulin-dependent diabetes mellitus (NIDDM) were matched by age with ten healthy volunteers each. DNA was extracted from mononuclear cells from whole blood. 8-OHdG was assayed by high-pressure liquid chromatography, and ROS were assayed by chemiluminescence. Findings IDDM and NIDDM patients had significantly higher median concentrations (p
ISSN:0140-6736
1474-547X
DOI:10.1016/S0140-6736(96)90013-6