Mobilization of iron from cellular ferritin by ascorbic acid in neuroblastoma SK-N-SH cells: an EPR study

The mobilization of iron from intracellular ferritin by ascorbic acid has been analysed in situ by electron paramagnetic resonance (EPR) spectroscopy. EPR enables a distinction between ferritins and other Fe 3+-binding cellular components. The ordered iron core of ferritin gives rise to a resonance...

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Bibliographic Details
Published in:FEBS letters 1996-02, Vol.381 (1), p.131-134
Main Authors: Baader, S.L., Bill, E., Trautwein, A.X., Bruchelt, G., Matzanke, B.F.
Format: Article
Language:English
Subjects:
Ascorbic acid
Ascorbic Acid - pharmacology
au, arbitrary units
Cell Line
Electron paramagnetic resonance
Electron Spin Resonance Spectroscopy
EPR, electron paramagnetic resonance
Ferritin
Ferritins - chemistry
Ferritins - drug effects
Ferritins - metabolism
Hot Temperature
Humans
Iron - metabolism
Iron release
Kinetics
Neuroblastoma
PBS, phosphate-buffered saline
Thermodynamics
Time Factors
Tumor Cells, Cultured
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Summary:The mobilization of iron from intracellular ferritin by ascorbic acid has been analysed in situ by electron paramagnetic resonance (EPR) spectroscopy. EPR enables a distinction between ferritins and other Fe 3+-binding cellular components. The ordered iron core of ferritin gives rise to a resonance signal which can be observed only at temperatures above 50 K. In the present study we clearly demonstrate that ascorbic acid is capable of mobilizing iron from ferritin in the cellular system by reduction of the ferric ion core in neuroblastoma SK-N-SH cells. This mechanism may open new ways in the therapy of this hardly curable tumor in stage IV, especially in combination with some cytostatic drugs.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00098-1