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Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner
Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in Saccharomyces cerevislae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a...
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| Published in: | FEBS letters 1996-03, Vol.382 (1), p.97-100 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_end_page | 100 |
| container_issue | 1 |
| container_start_page | 97 |
| container_title | FEBS letters |
| container_volume | 382 |
| creator | Yona, Eyal Bangio, Haim Friedman, Yael Shpungin, Sally Katcoff, Don J. |
| description | Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in
Saccharomyces cerevislae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes,
HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of
HO expression and the presence of the short sequence in situ in the
HO gene. By comparing the expression of
HO from wild type or XBS deleted
HO promoters, we concluded that XBS serves as a weak UAS in situ in the
HO gene, that it influences
HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of
SIN1 on
HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS. |
| doi_str_mv | 10.1016/0014-5793(96)00159-7 |
| format | article |
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Saccharomyces cerevislae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes,
HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of
HO expression and the presence of the short sequence in situ in the
HO gene. By comparing the expression of
HO from wild type or XBS deleted
HO promoters, we concluded that XBS serves as a weak UAS in situ in the
HO gene, that it influences
HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of
SIN1 on
HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(96)00159-7</identifier><identifier>PMID: 8612772</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>ABS ; Base Sequence ; beta-Galactosidase - biosynthesis ; beta-Galactosidase - genetics ; Chromosomal Proteins, Non-Histone - metabolism ; Chromosomal Proteins, Non-Histone - physiology ; Deoxyribonucleases, Type II Site-Specific - genetics ; DNA, Fungal - metabolism ; DNA-Binding Proteins - metabolism ; DNA-Binding Proteins - physiology ; Gene Expression Regulation, Fungal - genetics ; Genes, Reporter - genetics ; HMG1 ; HO transcription ; Molecular Sequence Data ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; SIN1 ; SWI/SNF complex ; Transcription Factors - physiology ; Transcription, Genetic - genetics ; wild type ; X-binding sequence</subject><ispartof>FEBS letters, 1996-03, Vol.382 (1), p.97-100</ispartof><rights>1996</rights><rights>FEBS Letters 382 (1996) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579396001597$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8612772$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yona, Eyal</creatorcontrib><creatorcontrib>Bangio, Haim</creatorcontrib><creatorcontrib>Friedman, Yael</creatorcontrib><creatorcontrib>Shpungin, Sally</creatorcontrib><creatorcontrib>Katcoff, Don J.</creatorcontrib><title>Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in
Saccharomyces cerevislae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes,
HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of
HO expression and the presence of the short sequence in situ in the
HO gene. By comparing the expression of
HO from wild type or XBS deleted
HO promoters, we concluded that XBS serves as a weak UAS in situ in the
HO gene, that it influences
HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of
SIN1 on
HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.</description><subject>ABS</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>beta-Galactosidase - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Chromosomal Proteins, Non-Histone - physiology</subject><subject>Deoxyribonucleases, Type II Site-Specific - genetics</subject><subject>DNA, Fungal - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Gene Expression Regulation, Fungal - genetics</subject><subject>Genes, Reporter - genetics</subject><subject>HMG1</subject><subject>HO transcription</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>SIN1</subject><subject>SWI/SNF complex</subject><subject>Transcription Factors - physiology</subject><subject>Transcription, Genetic - genetics</subject><subject>wild type</subject><subject>X-binding sequence</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFUcFu1DAUtBCoLIU_AMknBIeAHTtxfEEqqy6tVNEDcLZenJeuUeIstgPanvvhOLurHuFia97MG9kzhLzm7ANnvP7IGJdFpbR4p-v3GVS6UE_IijdKFELWzVOyepQ8Jy9i_Mkybrg-I2dNzUulyhV5WG8hgE0Y3D0kN3k69RRo3E4h0dm7XzPSiPn0FqnzNG2R7hFiole39A490l2YxinvZwoSDXg3D5AwLnwK4KMNbncwzttAv11_5bTDHfoOfaIjeI_hJXnWwxDx1ek-Jz82l9_XV8XN7Zfr9cVNgZJxVZRtW1bQqLKXXNUabGuV6LnVXVl1CmTDGtYqZpnOWEAHmMU5KhC9kLIU4py8PfrmN-cvxWRGFy0OA3ic5miU0lpL-X8hr2qmq4pl4ZuTcG5H7MwuuBHC3pzyzfzmyP9xA-4fac7MUqFZ-jFLP0YfQKWNMpvLz-VCLHNdH6YqG306GmHO57fDYKJ1SymdC2iT6Sb3T1fxF-wzpR4</recordid><startdate>19960311</startdate><enddate>19960311</enddate><creator>Yona, Eyal</creator><creator>Bangio, Haim</creator><creator>Friedman, Yael</creator><creator>Shpungin, Sally</creator><creator>Katcoff, Don J.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19960311</creationdate><title>Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner</title><author>Yona, Eyal ; Bangio, Haim ; Friedman, Yael ; Shpungin, Sally ; Katcoff, Don J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e4017-2bb25a872f41769acbc73f1c9d25d7a48080b70c0925d3adaea87101a3f344233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ABS</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>beta-Galactosidase - genetics</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Chromosomal Proteins, Non-Histone - physiology</topic><topic>Deoxyribonucleases, Type II Site-Specific - genetics</topic><topic>DNA, Fungal - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Gene Expression Regulation, Fungal - genetics</topic><topic>Genes, Reporter - genetics</topic><topic>HMG1</topic><topic>HO transcription</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>SIN1</topic><topic>SWI/SNF complex</topic><topic>Transcription Factors - physiology</topic><topic>Transcription, Genetic - genetics</topic><topic>wild type</topic><topic>X-binding sequence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yona, Eyal</creatorcontrib><creatorcontrib>Bangio, Haim</creatorcontrib><creatorcontrib>Friedman, Yael</creatorcontrib><creatorcontrib>Shpungin, Sally</creatorcontrib><creatorcontrib>Katcoff, Don J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yona, Eyal</au><au>Bangio, Haim</au><au>Friedman, Yael</au><au>Shpungin, Sally</au><au>Katcoff, Don J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-03-11</date><risdate>1996</risdate><volume>382</volume><issue>1</issue><spage>97</spage><epage>100</epage><pages>97-100</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in
Saccharomyces cerevislae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes,
HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of
HO expression and the presence of the short sequence in situ in the
HO gene. By comparing the expression of
HO from wild type or XBS deleted
HO promoters, we concluded that XBS serves as a weak UAS in situ in the
HO gene, that it influences
HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of
SIN1 on
HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8612772</pmid><doi>10.1016/0014-5793(96)00159-7</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1996-03, Vol.382 (1), p.97-100 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77999443 |
| source | ScienceDirect Journals |
| subjects | ABS Base Sequence beta-Galactosidase - biosynthesis beta-Galactosidase - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosomal Proteins, Non-Histone - physiology Deoxyribonucleases, Type II Site-Specific - genetics DNA, Fungal - metabolism DNA-Binding Proteins - metabolism DNA-Binding Proteins - physiology Gene Expression Regulation, Fungal - genetics Genes, Reporter - genetics HMG1 HO transcription Molecular Sequence Data Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins SIN1 SWI/SNF complex Transcription Factors - physiology Transcription, Genetic - genetics wild type X-binding sequence |
| title | Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner |
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