Cloning and sequencing of the dnaK operon of Bacillus stearothermophilus

Here, we report the cloning of a 5.8-kb PstI fragment of chromosomal DNA from Bacillus stearothermophilus (Bt) carrying the three genes, grpE, dnaK and dnaJ. This fragment contains, in addition, the 3′-end of an open reading frame which has been shown to be part of the dnaK operon in three bacterial...

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Bibliographic Details
Published in:Gene 1996-04, Vol.170 (1), p.81-84
Main Authors: Herbort, Markus, Schön, Ulrike, Angermann, Karin, Lang, Jens, Schumann, Wolfgang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus stearothermophilus
Bacterial Proteins - genetics
Base Sequence
Chromosomes, Bacterial - genetics
Cloning, Molecular
DNA, Bacterial - genetics
dnaJ
Escherichia coli Proteins
Genetic Complementation Test
Geobacillus stearothermophilus - genetics
grpE
heat-shock protein
Heat-Shock Proteins - genetics
HSP40 Heat-Shock Proteins
HSP70 Heat-Shock Proteins - genetics
molecular chaperone
Molecular Sequence Data
Open Reading Frames
Operon - genetics
Recombinant DNA
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Summary:Here, we report the cloning of a 5.8-kb PstI fragment of chromosomal DNA from Bacillus stearothermophilus (Bt) carrying the three genes, grpE, dnaK and dnaJ. This fragment contains, in addition, the 3′-end of an open reading frame which has been shown to be part of the dnaK operon in three bacterial species. The dnaJ gene could complement an Escherichia coli dnaJts mutant for growth at high temperature. Sequencing and hybridization data strongly suggest that the Bt chromosome contains an analog of dnaJ.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(95)00859-4