Detection of Apoptotic Cell Death by Proton Nuclear Magnetic Resonance Spectroscopy

Cells undergoing apoptosis (programmed cell death) display profound morphologic and biochemical changes in the nucleus, cytoplasm, and plasma membrane. We have shown a direct temporal relationship between the onset of apoptosis in Jurkat T-cell lymphoblast cultures and a greater than twofold increas...

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Bibliographic Details
Published in:Blood 1996-03, Vol.87 (5), p.1951-1956
Main Authors: Blankenberg, Francis G., Storrs, Richard W., Naumovski, Louie, Goralski, Thomas, Spielman, Daniel
Format: Article
Language:English
Subjects:
3T3 Cells - drug effects
Animals
Apoptosis - drug effects
Biological and medical sciences
Burkitt Lymphoma - pathology
Cell Line - drug effects
Culture Media, Serum-Free - pharmacology
Demecolcine - pharmacology
Dexamethasone - pharmacology
DNA Damage
Doxorubicin - pharmacology
HL-60 Cells - drug effects
HL-60 Cells - metabolism
Humans
Investigative techniques, diagnostic techniques (general aspects)
Leukemia - pathology
Lymphocyte Subsets - drug effects
Magnetic Resonance Spectroscopy
Medical sciences
Mice
Miscellaneous. Technology
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - genetics
Proto-Oncogene Proteins - biosynthesis
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins c-bcl-2
Protons
Radiodiagnosis. Nmr imagery. Nmr spectrometry
Tumor Cells, Cultured - drug effects
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Summary:Cells undergoing apoptosis (programmed cell death) display profound morphologic and biochemical changes in the nucleus, cytoplasm, and plasma membrane. We have shown a direct temporal relationship between the onset of apoptosis in Jurkat T-cell lymphoblast cultures and a greater than twofold increase in the signal intensity of the methylene resonance (at 1.3 ppm) as observed by proton nuclear magnetic resonance spectroscopy (1H NMR). The increase in the methylene resonance intensity was seen when apoptosis was induced by serum deprivation, glucocorticoid, and doxorubicin treatment but not in necrotic (nonapoptotic) cell death. We have found similar changes in a variety of other cell lines undergoing apoptosis including the Hut 78 T-cell leukemia, JY natural killer T-cell leukemia, Daudi B-cell lymphoma, HeLa, and 3T3 fibroblast cell lines. Furthermore, this spectral change was diminished in Bcl-2 overexpressing HL-60 cell cultures treated with doxorubicin, which were relatively resistant to apoptosis, as compared to apoptotic HL-60 cultures. 1H NMR spectroscopy therefore may be useful in detecting apoptotic cell death in vivo.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V87.5.1951.1951