Lysis of natural killer-sensitive and -resistant tumor cells by natural killer cytotoxic factors (NKCF)-containing liposomes

The lethal hit stage in NK cell-mediated lysis requires a complex series of events involving the release of NKCF, subsequent binding of these factors to the target cell, susceptibility of the target cell to lysis by NKCF.Binding of NKCF alone is not sufficient because a number of tumor cells are abl...

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Bibliographic Details
Published in:Immunobiology (1979) 1987-12, Vol.176 (1), p.35-46
Main Authors: Roozemond, Robert C., Urli, Dorothy C., Wright, Susan C., Graves, Scott S., Bonavida, Benjamin
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others)
Cytotoxicity, Immunologic
Fluoresceins
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunological reactions in vitro
Killer Cells, Natural - immunology
Killer Factors, Yeast
Liposomes - administration & dosage
Proteins - administration & dosage
Proteins - immunology
Tumor Cells, Cultured - immunology
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Summary:The lethal hit stage in NK cell-mediated lysis requires a complex series of events involving the release of NKCF, subsequent binding of these factors to the target cell, susceptibility of the target cell to lysis by NKCF.Binding of NKCF alone is not sufficient because a number of tumor cells are able to bind NKCF without being lysed, suggesting the need for an additional processing step active on susceptible target cells.In the present study, we show that the interaction with liposome-incorporated NKCF renders NK resistant target cells sensitive to NKCF-mediated lysis.These results suggest that NKCF may mediate their cytotoxic effects through internalization of these factors into the cytosol.
ISSN:0171-2985
1878-3279
DOI:10.1016/S0171-2985(87)80098-0