Phagocytosis and Intracellular Killing of Candida albicans by Macrophages Exposed to Myeloperoxidase

Candida albicans is an opportunistic pathogen whose resurgence coincides with the rising number of AIDS patients. Neutrophils are known to be involved in the clearance of Candida infections; however, the role of macrophages in host defenses against this organism is not well understood. The present s...

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Bibliographic Details
Published in:The Journal of infectious diseases 1996-05, Vol.173 (5), p.1202-1207
Main Authors: Lefkowitz, Stanley S., Gelderman, Monique P., Lefkowitz, Doris L., Moguilevsky, Nicole, Bollen, Alex
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - pharmacology
Animals
Biological and medical sciences
Candida albicans
Candida albicans - immunology
Catalase - pharmacology
Enzymes
Female
Free Radical Scavengers - pharmacology
Fundamental and applied biological sciences. Psychology
Infections
Inflammation
Intracellular killing
Macrophages
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - physiology
Major Articles
Male
Mice
Mice, Inbred C57BL
Microbiology
Mycology
Neutrophils
Pathogenicity, host-agent relations, miscellaneous strains, epidemiology
Peroxidase - pharmacology
Phagocytosis
Phagocytosis - drug effects
Reactive Oxygen Species
Receptors
Recombinant Proteins - pharmacology
Respiratory Burst
Superoxide Dismutase - pharmacology
Yeasts
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Summary:Candida albicans is an opportunistic pathogen whose resurgence coincides with the rising number of AIDS patients. Neutrophils are known to be involved in the clearance of Candida infections; however, the role of macrophages in host defenses against this organism is not well understood. The present study was undertaken to examine an unrecognized interaction between neutrophils and macrophages resulting in enhanced killing of candidae in vitro. Murine peritoneal macrophages exposed to recombinant myeloperoxidase exhibited enhancement of the respiratory burst, increased phagocytosis, and a dose-dependent increase in intracellular killing of Candida species. Radical scavengers reduced the killing, indicating a role of reactive oxygen intermediates in the candidacidal activity observed. These data suggest that at the site of infection, myeloperoxidase released from neutrophils activates macrophages and induces microbicidal activity.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/173.5.1202