In vitro Restoration of T Cell Immune Function in Human Immunodeficiency Virus-Positive Persons: Effects of Interleukin (IL)-12 and Anti-IL-10 [with Discussion]

Cells from human immunodeficiency virus (HIV)-positive patients were evaluated for their in vitro responsiveness to recall antigen, alloantigen, and phytohemagglutinin (PHA) following the in vitro addition of interleukin (IL)-12 or anti-IL-10. Three-color flow cytometric analysis of CD4 and CD8 subs...

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Bibliographic Details
Published in:The Journal of infectious diseases 1996-05, Vol.173 (5), p.1085-1091
Main Authors: Landay, Alan L., Clerici, Mario, Hashemi, Farah, Kessler, Harold, Berzofsky, Jay A., Shearer, Gene M.
Format: Article
Language:English
Subjects:
AIDS
AIDS/HIV
Antibodies
Antigens
Biological and medical sciences
CD4 Lymphocyte Count
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cytokines
HIV
HIV - immunology
HIV Infections - immunology
human immunodeficiency virus
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Influenza A virus
Influenza A virus - immunology
Interleukin-10 - immunology
Interleukin-12 - immunology
Isoantigens
Isoantigens - immunology
Lymphocyte Activation - immunology
Lymphocyte Subsets
Major Articles
Medical sciences
Mitogens - immunology
Phytohemagglutinins - immunology
Reagents
T lymphocytes
T-Lymphocytes - immunology
Viral Envelope Proteins - immunology
Viruses
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Summary:Cells from human immunodeficiency virus (HIV)-positive patients were evaluated for their in vitro responsiveness to recall antigen, alloantigen, and phytohemagglutinin (PHA) following the in vitro addition of interleukin (IL)-12 or anti-IL-10. Three-color flow cytometric analysis of CD4 and CD8 subsets was done to determine whether specific in vivo alterations in cell surface markers are associated with in vitro function changes. The results demonstrated a hierarchical response pattern to recall antigens versus alloantigen versus PHA, and these in vitro responses were associated with the number and activation status of CD4 cells. The in vitro addition of IL-12 or anti-IL-10 could restore antigen responses (HIV envelope peptides or influenza) in patients with 200-500 CD4 cells/µL; however, in patients with
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/173.5.1085