The effect of antisecretory factor on the permeability of nerve cell membrane to chloride ion

The antisecretory factor (ASF) is a hormone-like protein (m.w. 60,000) that most effectively counteracts hypersecretion in vivo in the small intestine of pigs and rats. The present report demonstrate that 10(-13) moles of ASF inhibits significantly the 36Cl- permeation through the isolated neuronal...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 1987-12, Vol.410 (6), p.648-651
Main Authors: LANGE, S, LONNROTH, I, PALM, A, HYDEN, H
Format: Article
Language:English
Subjects:
Animals
Antidiarrheals - pharmacology
Applied sciences
Cell Membrane Permeability - drug effects
Chlorides - pharmacokinetics
Exact sciences and technology
In Vitro Techniques
Neuroglia - drug effects
Neuroglia - metabolism
Neuropeptides - pharmacology
Other techniques and industries
Rabbits
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The antisecretory factor (ASF) is a hormone-like protein (m.w. 60,000) that most effectively counteracts hypersecretion in vivo in the small intestine of pigs and rats. The present report demonstrate that 10(-13) moles of ASF inhibits significantly the 36Cl- permeation through the isolated neuronal plasma membrane of Deiters' cells in rabbits. This effect was enhanced by 0.2 mM gamma-aminobutyric acid (GABA), and quenched by the addition of anti-ASF immunoglobulins; pretreatment of the neuronal membrane with nipecotic acid (10(-6) M) or with bicuculline (10(-3) M) abolished the ASF action whilst picrotoxin (10(-4) M) pretreatment left the inhibitory effect of ASF unaffected. The results suggest that ASF blocks chloride channels in neuronal membranes, including those channels activated by GABA.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00581326