Augmentation of Killer Activity of Murine Spleen Cells against Allogeneic and Syngeneic Tumor Cells by Inoculation of Alloantigen in vivo

After C57BL/6 (B6) mice were inoculated with BALB/c spleen cells via tail vein, kinetics of cytotoxic activities in the B6 mice against sensitizing alloantigens (H-2d) and against syngeneic antigens were investigated using, as target cells, P815 mastocytoma cells (H-2d) and B16 melanoma cells (H-2b)...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 1987, Vol.31(12), pp.1245-1254
Main Authors: Nishi, Yasuaki, Hosokawa, Tomohide, Aoike, Akira, Kamahora, Takeshi, Sudo, Hiromasa, Kawai, Keiichi
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface - immunology
Applied sciences
Epitopes - immunology
Exact sciences and technology
H-2 Antigens - immunology
Immunization
In Vitro Techniques
Killer Cells, Natural - immunology
Mice
Mice, Inbred Strains
Neoplasms, Experimental - immunology
Other techniques and industries
Phenotype
Spleen - immunology
Time Factors
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Summary:After C57BL/6 (B6) mice were inoculated with BALB/c spleen cells via tail vein, kinetics of cytotoxic activities in the B6 mice against sensitizing alloantigens (H-2d) and against syngeneic antigens were investigated using, as target cells, P815 mastocytoma cells (H-2d) and B16 melanoma cells (H-2b). Cytotoxic activity against P815 in the B6 spleen cells reached a peak 3 days after alloantigen inoculation, decreased drastically on day 5 and rose again thereafter. The profile of anti-B16 cytotoxic activity was similar to that of anti-P815 activity. The cytotoxic activity against P815 was inhibited partially by cold B16, but that against B16 was not inhibited by cold P815. Surface phenotype of cytotoxic cells against P815 was Lyt2+, Thyl+, Asialo GM1+ and that of cytotoxic cells against B16 was Lyt2-, Thyl+/-, and Asialo GM1+. The results indicate that inoculation of B6 mice with allogeneic BALB/c spleen cells induce two types of cytotoxic cells; one is similar to lymphokine-activated killer (LAK) cells and the other is activated natural killer cells.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1987.tb01357.x