Relative Contribution of the Selectins in the Neutrophil Recruitment Caused by the Chemokine Cytokine-Induced Neutrophil Chemoattractant (CINC)

• Published in: Biochemical and biophysical research communications 1996-04, Vol.221 (3), p.692-696
• Main Authors: Harris, Jeanette G., Flower, Roderick J., Watanabe, Kazuyoshi, Tsurufuji, Susumu, Wolitzky, Barry A., Perretti, Mauro
• Format: Article
• Language: English
• Subjects: Animals; Cell Movement - physiology; Chemokine CXCL1; Chemokines, CXC; Chemotactic Factors - physiology; Growth Substances - physiology; Histamine - physiology; Intercellular Signaling Peptides and Proteins; Male; Mice; Neutrophils - cytology; Platelet Activating Factor - physiology; Rats; Selectins - physiology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.0658

Rat CINC induced a dose- and time-dependent accumulation of neurophils into murine air-pouches, a response which was inhibited by two selective H1-antagonists, mepyramine and triprolidine (∼60%). As pretreatment with fucoidin abolished CINC effect, the relative time-related contribution of selectins on this process was then investigated by using specific monoclonal antibodies (mAb). Anti-CD62L mAb gave a similar degree of inhibition of CINC-induced cell accumulation both at the 2h and 4h time-point (∼75%). Anti-CD62P mAb, but not the anti-CD62E mAb, inhibited PMN accumulation at 2h (65%), but only co-administration of these two mAbs inhibited the cell response to CINC at the 4h time-point (90%). Thus endogenous histamine, CD62L, CD62P, and CD62E, though to a different degree, are required for PMN extravasation observed in response to CINC administration.