DNA Replication Fork Pause Sites Dependent on Transcription

Replication fork pause (RFP) sites transiently arresting replication fork movement were mapped to transfer RNA (tRNA) genes of Saccharomyces cerevisiae in vivo. RFP sites are polar, stalling replication forks only when they oppose the direction of tRNA transcription. Mutant tRNA genes defective in a...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1996-05, Vol.272 (5264), p.1030-1033
Main Authors: Deshpande, Atul M., Newlon, Carol S.
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Climate
Cloning, Molecular
Deoxyribonucleic acid
DNA
DNA Replication
Fractions
Fundamental and applied biological sciences. Psychology
Gels
Genes
Genes, Fungal
Genetic aspects
Genetic transcription
Genetics
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutation
Plasmids
Point Mutation
Polar regions
Repetitive Sequences, Nucleic Acid
Replication
Replication Origin
Ribonucleic acid
RNA
RNA Polymerase III - metabolism
RNA, Fungal - genetics
RNA, Transfer - genetics
rRNA genes
Saccharomyces
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Sequence Deletion
Temperature
Transcription (Genetics)
Transcription Factors - metabolism
Transcription initiation site
Transcription, Genetic
Transfer RNA
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Description
Summary:Replication fork pause (RFP) sites transiently arresting replication fork movement were mapped to transfer RNA (tRNA) genes of Saccharomyces cerevisiae in vivo. RFP sites are polar, stalling replication forks only when they oppose the direction of tRNA transcription. Mutant tRNA genes defective in assembly of transcription initiation complexes and a temperature-sensitive RNA polymerase III mutant (rpc160-41) defective in initiation of transcription do not stall replication forks, suggesting that transcription is required for RFP activity.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.272.5264.1030