Unusual DNA structures

In vitro investigations with recombinant plasmids, short synthetic oligonucleotides, and restriction fragments have provided substantial insights into the types of sequences that can adopt each of these DNA structures. Other studies have identified the conditions which stabilize these structures, th...

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Bibliographic Details
Published in:The Journal of biological chemistry 1988-01, Vol.263 (3), p.1095-1098
Main Author: Wells, R D
Format: Article
Language:English
Subjects:
Biological and medical sciences
DNA - analysis
DNA, Bacterial - analysis
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Molecular biophysics
Nucleic Acid Conformation
Repetitive Sequences, Nucleic Acid
Structure in molecular biology
Temperature
Tridimensional structure
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Summary:In vitro investigations with recombinant plasmids, short synthetic oligonucleotides, and restriction fragments have provided substantial insights into the types of sequences that can adopt each of these DNA structures. Other studies have identified the conditions which stabilize these structures, the mechanisms and kinetics of formation, the effect of methylation of cytosine residues, the properties of junction interfaces, immunological properties, characteristics of proteins which bind, and interactions with small ligands. Structural questions with macromolecules such as proteins or nucleic acids are solved by physical analyses. Genetic analyses may be required in order to not perturb cellular superstructures and functions. Two very recent contributions describe biochemical-genetic analyses that demonstrate the in vivo existence of left-handed DNA and cruciforms.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)57268-4