Identification of nonadherent mononuclear cells in human cord blood that differentiate into macrophages

We describe a population of nonadherent cells in neonatal cord blood that, upon in vitro cultivation, develop into monocyte‐macrophages. These cells initially are negative for nonspecific esterase cytoplasmic activity, lack the monocyte marker MO.2, fall into smaller, nonmonocytic cell size areas, a...

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Bibliographic Details
Published in:Journal of leukocyte biology 1988-01, Vol.43 (1), p.51-59
Main Authors: F Santiago-Schwarz, H B Fleit
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcitriol - pharmacology
Cell Adhesion
Cell Differentiation
Cell Separation
Cells, Cultured
cord blood
FACS
Fetal Blood - cytology
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Infant, Newborn
Leukocytes, Mononuclear - cytology
Lipopolysaccharides - pharmacology
macrophage differentiation
Macrophages - cytology
Myeloid cells: ontogeny, maturation, markers, receptors
Nylons
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Summary:We describe a population of nonadherent cells in neonatal cord blood that, upon in vitro cultivation, develop into monocyte‐macrophages. These cells initially are negative for nonspecific esterase cytoplasmic activity, lack the monocyte marker MO.2, fall into smaller, nonmonocytic cell size areas, as determined by fluorescence‐activated cell sorter (FACS)‐assisted size analysis, and differentiate into macrophages under nonstimulatory culture conditions (in the absence of exogenous colony stimulating factors,
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.43.1.51