Determination and characterization of chloroguaiacol conjugates in fish bile by HPLC

In this study a small-scale technique for direct analysis of metabolic conjugates of 4,5,6-trichloro- (CG-3) and tetrachloroguaiacols (CG-4) in fish bile by RP-HPLC is presented. Only one metabolite, glucuronic acid conjugate, was demonstrated in two Lake Baikal fishes ( Leuciscus leuciscus baikalen...

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Bibliographic Details
Published in:Environmental pollution (1987) 1988, Vol.55 (2), p.79-87
Main Authors: Oikari, A.O.J., Baram, G.I., Evstafyev, V.K., Grachev, M.A.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
bile
Biological and medical sciences
characterization
chlorinated phenolics
determination
Ecotoxicology, biological effects of pollution
fish
Fresh water environment
Fundamental and applied biological sciences. Psychology
metabolic conjugates
phenols
pulp and paper mill effluents
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Summary:In this study a small-scale technique for direct analysis of metabolic conjugates of 4,5,6-trichloro- (CG-3) and tetrachloroguaiacols (CG-4) in fish bile by RP-HPLC is presented. Only one metabolite, glucuronic acid conjugate, was demonstrated in two Lake Baikal fishes ( Leuciscus leuciscus baikalensis and Cottus kessleri) exposed to CG-3 or CG-4 at 6°C for 1–2 days. In Leuciscus the ratio between free CG-4 in the ambient water and conjugated CG-4 in the bile averaged 264 000. Intraperitoneal injections of tetrachloroveratrol (CV-4) into Thymallus arcticus baikalensis did not reveal any traces of free CV-4 or CG-4 glucuronide in the bile in 2 days at 5°C. We therefore suggest that CV-4 was not demethylated and subsequently conjugated with glucuronic acid in the liver of this fish species.
ISSN:0269-7491
1873-6424
DOI:10.1016/0269-7491(88)90119-4