Changes in cyclic nucleotides during the calcium paradox in the isolated rat heart

Reperfusion of hearts with a Ca2+-containing medium after a perfusion period in Ca2+-free medium results in irreversible cell damage (calcium paradox). In this investigation we have studied coronary flow and cyclic AMP and cyclic GMP levels after several periods of Ca2+-free perfusion in isolated ra...

Full description

Saved in:
Bibliographic Details
Published in:Pflügers Archiv 1987-12, Vol.410 (6), p.657-663
Main Authors: KARAKULLUKCU, Y. E, OZCELIK, T, GOKHAN, N
Format: Article
Language:English
Subjects:
Acetylcholine - pharmacology
Animals
Biological and medical sciences
Calcium - pharmacology
Coronary Circulation - drug effects
Cyclic AMP - metabolism
Cyclic GMP - metabolism
Fundamental and applied biological sciences. Psychology
Heart
In Vitro Techniques
Male
Myocardial Contraction - drug effects
Myocardium - metabolism
Norepinephrine - pharmacology
Papaverine - pharmacology
Perfusion
Phosphates - pharmacology
Rats
Rats, Inbred Strains
Vertebrates: cardiovascular system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Reperfusion of hearts with a Ca2+-containing medium after a perfusion period in Ca2+-free medium results in irreversible cell damage (calcium paradox). In this investigation we have studied coronary flow and cyclic AMP and cyclic GMP levels after several periods of Ca2+-free perfusion in isolated rat hearts. We also investigated the effects of papaverine (Pap), noradrenaline (NA), acetylcholine (ACh) and absence of inorganic phosphate during Ca2+-free perfusion on coronary flow (CF) and cyclic nucleotide levels. Inability of the heart to recover contractile activity with development of contracture during the reperfusion period was accepted as indicative of the calcium paradox. Ca2+-free perfusion alone and NA and absence of inorganic phosphate during the Ca2+-free perfusion period increased CF, whereas Pap and ACh decreased it. However, only Ca2+-free perfusion and NA elevated cyclic AMP. On the other hand, Pap and ACh increased cyclic GMP (with a transient rise of cyclic AMP in Pap infusion), and absence of inorganic phosphate decreased both cyclic AMP and cyclic GMP. Pap, ACh and absence of phosphate prevented the calcium paradox. Our study suggests that increased cyclic AMP during the Ca2+-free perfusion may contribute, with the other factors, to the occurrence of the calcium paradox.
ISSN:0031-6768
1432-2013
DOI:10.1007/bf00581328