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A Point Mutation in HLA-A0201 Results in Failure to Bind the TAP Complex and to Present Virus-Derived Peptides to CTL
Mutating the HLA-A*0201 heavy chain from threonine to lysine at position 134 (T134K) results in a molecule that presents exogenous peptide, but cannot present endogenously derived antigen. This is reflected in diminished cell surface expression and altered intracellular trafficking of T134K. The fai...
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Published in: | Immunity (Cambridge, Mass.) Mass.), 1996-05, Vol.4 (5), p.505-514 |
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container_title | Immunity (Cambridge, Mass.) |
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creator | Peace-Brewer, Amy L Tussey, Lynda G Matsui, Masanori Li, Guoxuan Quinn, Daniel G Frelinger, Jeffrey A |
description | Mutating the HLA-A*0201 heavy chain from threonine to lysine at position 134 (T134K) results in a molecule that presents exogenous peptide, but cannot present endogenously derived antigen. This is reflected in diminished cell surface expression and altered intracellular trafficking of T134K. The failure of T134K to present endogenous antigen can be overcome by using an ER targeting sequence, suggesting that the antigen presentation defect is restricted to TAP-dependent peptide loading. The ability of T134K to load peptide in a TAP-dependent manner is dramatically reduced compared with HLA-A*0201. By coimmunoprecipitation there is no detectable association of the T134K molecule with the TAP complex. Thus, T134K selectively affects TAP association and peptide loading, suggesting a requirement for the direct interaction of MHC class I heavy chain and the TAP complex for efficient presentation of endogenous antigen. |
doi_str_mv | 10.1016/S1074-7613(00)80416-1 |
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Tussey, Lynda G ; Matsui, Masanori ; Li, Guoxuan ; Quinn, Daniel G ; Frelinger, Jeffrey A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-f0061ef3f47de3cb4339d74492db98aad69f22788f1ad19dd39931daa7d03bc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Antigen Presentation - genetics</topic><topic>Antigens, Viral - immunology</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Biological Transport - genetics</topic><topic>Biological Transport - immunology</topic><topic>Cell Line</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>HLA-A Antigens - chemistry</topic><topic>HLA-A Antigens - genetics</topic><topic>HLA-A Antigens - metabolism</topic><topic>Humans</topic><topic>Influenza A virus - immunology</topic><topic>Intracellular Fluid - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Peptides - metabolism</topic><topic>Point Mutation - immunology</topic><topic>Protein Binding - genetics</topic><topic>Protein Binding - immunology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Threonine - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peace-Brewer, Amy L</creatorcontrib><creatorcontrib>Tussey, Lynda G</creatorcontrib><creatorcontrib>Matsui, Masanori</creatorcontrib><creatorcontrib>Li, Guoxuan</creatorcontrib><creatorcontrib>Quinn, Daniel G</creatorcontrib><creatorcontrib>Frelinger, Jeffrey A</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunity (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peace-Brewer, Amy L</au><au>Tussey, Lynda G</au><au>Matsui, Masanori</au><au>Li, Guoxuan</au><au>Quinn, Daniel G</au><au>Frelinger, Jeffrey A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Point Mutation in HLA-A0201 Results in Failure to Bind the TAP Complex and to Present Virus-Derived Peptides to CTL</atitle><jtitle>Immunity (Cambridge, Mass.)</jtitle><addtitle>Immunity</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>4</volume><issue>5</issue><spage>505</spage><epage>514</epage><pages>505-514</pages><issn>1074-7613</issn><eissn>1097-4180</eissn><abstract>Mutating the HLA-A*0201 heavy chain from threonine to lysine at position 134 (T134K) results in a molecule that presents exogenous peptide, but cannot present endogenously derived antigen. This is reflected in diminished cell surface expression and altered intracellular trafficking of T134K. The failure of T134K to present endogenous antigen can be overcome by using an ER targeting sequence, suggesting that the antigen presentation defect is restricted to TAP-dependent peptide loading. The ability of T134K to load peptide in a TAP-dependent manner is dramatically reduced compared with HLA-A*0201. By coimmunoprecipitation there is no detectable association of the T134K molecule with the TAP complex. Thus, T134K selectively affects TAP association and peptide loading, suggesting a requirement for the direct interaction of MHC class I heavy chain and the TAP complex for efficient presentation of endogenous antigen.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8630735</pmid><doi>10.1016/S1074-7613(00)80416-1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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source | BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS |
subjects | Amino Acid Sequence Antigen Presentation - genetics Antigens, Viral - immunology ATP-Binding Cassette Transporters - metabolism Biological Transport - genetics Biological Transport - immunology Cell Line Endoplasmic Reticulum - metabolism HLA-A Antigens - chemistry HLA-A Antigens - genetics HLA-A Antigens - metabolism Humans Influenza A virus - immunology Intracellular Fluid - metabolism Molecular Sequence Data Peptides - metabolism Point Mutation - immunology Protein Binding - genetics Protein Binding - immunology T-Lymphocytes, Cytotoxic - immunology Threonine - genetics Transfection |
title | A Point Mutation in HLA-A0201 Results in Failure to Bind the TAP Complex and to Present Virus-Derived Peptides to CTL |
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