Inducible nitric oxide synthase in uterine smooth muscle

The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated ra...

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Bibliographic Details
Published in:Life sciences (1973) 1996, Vol.58 (13), p.PL249-PL255
Main Authors: Nakaya, Yutaka, Yamamoto, Satoshi, Hamada, Yoko, Kamada, Masaharu, Aono, Toshihiro, Niwa, Mineo
Format: Article
Language:English
Subjects:
Animals
Arginine - pharmacology
Dexamethasone - pharmacology
Female
Gene Expression - drug effects
inducible nitric oxide synthase
Isoenzymes - biosynthesis
Kidney - enzymology
Kinetics
lipopolysaccharide
Lipopolysaccharides - pharmacology
Liver - enzymology
Lung - enzymology
Macrophages - enzymology
Muscle, Smooth - drug effects
Muscle, Smooth - enzymology
nitric oxide
Nitric Oxide Synthase - biosynthesis
Organ Specificity
Polymerase Chain Reaction
Rats
Rats, Wistar
RNA, Messenger - biosynthesis
uterine smooth muscle
Uterus - drug effects
Uterus - enzymology
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Summary:The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated rats, low levels of iNOS mRNA was detected in the uterus and lungs, but absent or negligible in the kidneys and liver. NO was produced in the LPS-treated uterus by addition of 1 to 1000μM L-arginine. The production of NO in uterine tissue that faces the outside of the body may provide a bacteriocidal protective function against microorganisms in physiological condition. However, NO produced in a large amounts by cytokine and LPS may play some pathological reaction during septic shock or infection.
ISSN:0024-3205
1879-0631
DOI:10.1016/0024-3205(96)00064-1