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Inducible nitric oxide synthase in uterine smooth muscle
The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated ra...
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| Published in: | Life sciences (1973) 1996, Vol.58 (13), p.PL249-PL255 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c357t-f49f8cf45ccbeba9d3004d184ad9c2a9af92004910fcd4d7efc1db5e5db8c9d23 |
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| cites | cdi_FETCH-LOGICAL-c357t-f49f8cf45ccbeba9d3004d184ad9c2a9af92004910fcd4d7efc1db5e5db8c9d23 |
| container_end_page | PL255 |
| container_issue | 13 |
| container_start_page | PL249 |
| container_title | Life sciences (1973) |
| container_volume | 58 |
| creator | Nakaya, Yutaka Yamamoto, Satoshi Hamada, Yoko Kamada, Masaharu Aono, Toshihiro Niwa, Mineo |
| description | The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon
in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated rats, low levels of iNOS mRNA was detected in the uterus and lungs, but absent or negligible in the kidneys and liver. NO was produced in the LPS-treated uterus by addition of 1 to 1000μM L-arginine. The production of NO in uterine tissue that faces the outside of the body may provide a bacteriocidal protective function against microorganisms in physiological condition. However, NO produced in a large amounts by cytokine and LPS may play some pathological reaction during septic shock or infection. |
| doi_str_mv | 10.1016/0024-3205(96)00064-1 |
| format | article |
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in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated rats, low levels of iNOS mRNA was detected in the uterus and lungs, but absent or negligible in the kidneys and liver. NO was produced in the LPS-treated uterus by addition of 1 to 1000μM L-arginine. The production of NO in uterine tissue that faces the outside of the body may provide a bacteriocidal protective function against microorganisms in physiological condition. However, NO produced in a large amounts by cytokine and LPS may play some pathological reaction during septic shock or infection.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(96)00064-1</identifier><identifier>PMID: 8622552</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Arginine - pharmacology ; Dexamethasone - pharmacology ; Female ; Gene Expression - drug effects ; inducible nitric oxide synthase ; Isoenzymes - biosynthesis ; Kidney - enzymology ; Kinetics ; lipopolysaccharide ; Lipopolysaccharides - pharmacology ; Liver - enzymology ; Lung - enzymology ; Macrophages - enzymology ; Muscle, Smooth - drug effects ; Muscle, Smooth - enzymology ; nitric oxide ; Nitric Oxide Synthase - biosynthesis ; Organ Specificity ; Polymerase Chain Reaction ; Rats ; Rats, Wistar ; RNA, Messenger - biosynthesis ; uterine smooth muscle ; Uterus - drug effects ; Uterus - enzymology</subject><ispartof>Life sciences (1973), 1996, Vol.58 (13), p.PL249-PL255</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-f49f8cf45ccbeba9d3004d184ad9c2a9af92004910fcd4d7efc1db5e5db8c9d23</citedby><cites>FETCH-LOGICAL-c357t-f49f8cf45ccbeba9d3004d184ad9c2a9af92004910fcd4d7efc1db5e5db8c9d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8622552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakaya, Yutaka</creatorcontrib><creatorcontrib>Yamamoto, Satoshi</creatorcontrib><creatorcontrib>Hamada, Yoko</creatorcontrib><creatorcontrib>Kamada, Masaharu</creatorcontrib><creatorcontrib>Aono, Toshihiro</creatorcontrib><creatorcontrib>Niwa, Mineo</creatorcontrib><title>Inducible nitric oxide synthase in uterine smooth muscle</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon
in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated rats, low levels of iNOS mRNA was detected in the uterus and lungs, but absent or negligible in the kidneys and liver. NO was produced in the LPS-treated uterus by addition of 1 to 1000μM L-arginine. The production of NO in uterine tissue that faces the outside of the body may provide a bacteriocidal protective function against microorganisms in physiological condition. However, NO produced in a large amounts by cytokine and LPS may play some pathological reaction during septic shock or infection.</description><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Dexamethasone - pharmacology</subject><subject>Female</subject><subject>Gene Expression - drug effects</subject><subject>inducible nitric oxide synthase</subject><subject>Isoenzymes - biosynthesis</subject><subject>Kidney - enzymology</subject><subject>Kinetics</subject><subject>lipopolysaccharide</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Liver - enzymology</subject><subject>Lung - enzymology</subject><subject>Macrophages - enzymology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - enzymology</subject><subject>nitric oxide</subject><subject>Nitric Oxide Synthase - biosynthesis</subject><subject>Organ Specificity</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - biosynthesis</subject><subject>uterine smooth muscle</subject><subject>Uterus - drug effects</subject><subject>Uterus - enzymology</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMo67r6DxR6Ej1UkzRpm4sgix8LC170HNrJhI30Y01acf-9Kbt49DTMO--8wzyEXDJ6xyjL7ynlIs04lTcqv6WU5iJlR2TOykKlNM_YMZn_WU7JWQif0SRlkc3IrMw5l5LPSbnqzAiubjDp3OAdJP2PM5iEXTdsqoCJ65JxQO-6qLV9P2ySdgzQ4Dk5sVUT8OJQF-Tj-el9-Zqu315Wy8d1CpkshtQKZUuwQgLUWFfKZJQKw0pRGQW8UpVVPCqKUQtGmAItMFNLlKYuQRmeLcj1Pnfr-68Rw6BbFwCbpuqwH4MuSioKQbNoFHsj-D4Ej1ZvvWsrv9OM6gmYnmjoiYZWUxOBaRbXrg75Y92i-Vs6EIrzh_0c45PfDr0O4LADNM4jDNr07v8Dv49BeqU</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Nakaya, Yutaka</creator><creator>Yamamoto, Satoshi</creator><creator>Hamada, Yoko</creator><creator>Kamada, Masaharu</creator><creator>Aono, Toshihiro</creator><creator>Niwa, Mineo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Inducible nitric oxide synthase in uterine smooth muscle</title><author>Nakaya, Yutaka ; Yamamoto, Satoshi ; Hamada, Yoko ; Kamada, Masaharu ; Aono, Toshihiro ; Niwa, Mineo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-f49f8cf45ccbeba9d3004d184ad9c2a9af92004910fcd4d7efc1db5e5db8c9d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Arginine - pharmacology</topic><topic>Dexamethasone - pharmacology</topic><topic>Female</topic><topic>Gene Expression - drug effects</topic><topic>inducible nitric oxide synthase</topic><topic>Isoenzymes - biosynthesis</topic><topic>Kidney - enzymology</topic><topic>Kinetics</topic><topic>lipopolysaccharide</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Liver - enzymology</topic><topic>Lung - enzymology</topic><topic>Macrophages - enzymology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - enzymology</topic><topic>nitric oxide</topic><topic>Nitric Oxide Synthase - biosynthesis</topic><topic>Organ Specificity</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - biosynthesis</topic><topic>uterine smooth muscle</topic><topic>Uterus - drug effects</topic><topic>Uterus - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakaya, Yutaka</creatorcontrib><creatorcontrib>Yamamoto, Satoshi</creatorcontrib><creatorcontrib>Hamada, Yoko</creatorcontrib><creatorcontrib>Kamada, Masaharu</creatorcontrib><creatorcontrib>Aono, Toshihiro</creatorcontrib><creatorcontrib>Niwa, Mineo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakaya, Yutaka</au><au>Yamamoto, Satoshi</au><au>Hamada, Yoko</au><au>Kamada, Masaharu</au><au>Aono, Toshihiro</au><au>Niwa, Mineo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inducible nitric oxide synthase in uterine smooth muscle</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1996</date><risdate>1996</risdate><volume>58</volume><issue>13</issue><spage>PL249</spage><epage>PL255</epage><pages>PL249-PL255</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>The expression of inducible nitric oxide synthase (iNOS) mRNA in rat uterus upon
in vivo stimulation with lipopolysaccharide (LPS) was studied by reverse transcription and polymerase chain reaction. The injection of LPS induced an increase in mRNA levels of a macrophage-type iNOS. In unstimulated rats, low levels of iNOS mRNA was detected in the uterus and lungs, but absent or negligible in the kidneys and liver. NO was produced in the LPS-treated uterus by addition of 1 to 1000μM L-arginine. The production of NO in uterine tissue that faces the outside of the body may provide a bacteriocidal protective function against microorganisms in physiological condition. However, NO produced in a large amounts by cytokine and LPS may play some pathological reaction during septic shock or infection.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>8622552</pmid><doi>10.1016/0024-3205(96)00064-1</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 1996, Vol.58 (13), p.PL249-PL255 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78047403 |
| source | ScienceDirect Freedom Collection |
| subjects | Animals Arginine - pharmacology Dexamethasone - pharmacology Female Gene Expression - drug effects inducible nitric oxide synthase Isoenzymes - biosynthesis Kidney - enzymology Kinetics lipopolysaccharide Lipopolysaccharides - pharmacology Liver - enzymology Lung - enzymology Macrophages - enzymology Muscle, Smooth - drug effects Muscle, Smooth - enzymology nitric oxide Nitric Oxide Synthase - biosynthesis Organ Specificity Polymerase Chain Reaction Rats Rats, Wistar RNA, Messenger - biosynthesis uterine smooth muscle Uterus - drug effects Uterus - enzymology |
| title | Inducible nitric oxide synthase in uterine smooth muscle |
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