Intracellular free Ca2+ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells

This study documents a difference between cystic fibrosis human (CF‐HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca2+]i was abnormally reduced in CF‐HTG cells. The magnitude of the [Ca2+]i peak rise in r...

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Bibliographic Details
Published in:FEBS letters 1996-05, Vol.386 (2-3), p.123-127
Main Authors: Jacquot, Jacky, Maizières, Michaël, Spilmont, Christophe, Millot, Jean-Marc, Sébille, Stéphane, Merten, Marc, Kammouni, Wafa, Manfait, Michel
Format: Article
Language:English
Subjects:
[Ca2+]i
adenosine cyclic 3′,5′-monophosphate
Calcimycin - analogs & derivatives
Calcimycin - pharmacology
Calcium - metabolism
cAMP
CCD
Cells, Cultured
charge coupled device
Cystic fibrosis
Cystic Fibrosis - metabolism
Cystic Fibrosis - pathology
Exocytosis
Histamine - pharmacology
HNE
human neutrophil elastase
Human tracheal gland cell
Humans
Indo 1-AM
Indo-1 acetoxymethyl ester
Intracellular Ca2
intracellular free calcium ion concentration(s)
Ionophores - pharmacology
Leukocyte Elastase
Pancreatic Elastase - pharmacology
Trachea - cytology
Trachea - drug effects
Trachea - metabolism
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Summary:This study documents a difference between cystic fibrosis human (CF‐HTG) and normal human (HTG) tracheal gland cells: the ability of histamine to induce an increase of intracellular free calcium concentration [Ca2+]i was abnormally reduced in CF‐HTG cells. The magnitude of the [Ca2+]i peak rise in response to histamine is smaller in CF‐HTG cells than in HTG cells, and the percentage of CF‐HTG cells that increase [Ca2+]i is decreased compared with HTG cells. In contrast to histamine, the human neutrophil elastase (HNE) stimulation of both CF‐HTG and HTG cells generated [Ca2+]i asynchronous oscillations and the magnitude of the peak [Ca2+]i response as well as the percentage of responding cells were similar for both groups. By videomicroscopy observations, the secretory response (exocytosis of secretion granules) of CF‐HTG cells occurred with HNE, but not with histamine, thus suggesting that [Ca2+]i asynchronous oscillations may be linked to the exocytosis process in human tracheal gland cells.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00405-X