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High-performance liquid chromatographic determination of the biologically active principle hypericin in phytotherapeutic vegetable extracts and alcoholic beverages
Hypericin was determined using an RP C18 (3 μm) column (8.3 × 0.4 cm I.D.), thermostated at 50 °C. The separation was achieved with programmed elution using phosphate buffer (pH 7)-methanol (3:7) and water-methanol (3:7) as eluents. Fluorimetric detection was carried out with excitation at 470 nm an...
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Published in: | Journal of Chromatography A 1996-04, Vol.731 (1-2), p.336-339 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Hypericin was determined using an RP C18 (3 μm) column (8.3 × 0.4 cm I.D.), thermostated at 50 °C. The separation was achieved with programmed elution using phosphate buffer (pH 7)-methanol (3:7) and water-methanol (3:7) as eluents. Fluorimetric detection was carried out with excitation at 470 nm and emission at 590 nm. The analytical sample was prepared by simple dilution in methanol of the phytotherapeutic extract or of the alcoholic beverage. Hypericin can be rapidly and accurately determined at concentrations down to 0.1 mg/kg without any interferences. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/0021-9673(95)01222-2 |