B-Type Natriuretic Peptide Isolated from Frog Cardiac Ventricles

A new natriuretic peptide with 27 amino acid residues has been isolated from cardiac ventricles of the bullfrog,Rana catesbeiana.Since this ventricular peptide had high sequence identity to B-type (brain) natriuretic peptide (BNP), especially to chicken BNP (74%), we named it bullfrog BNP. Thus, sem...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-05, Vol.222 (2), p.323-329
Main Authors: Fukuzawa, A., Watanabe, T.X., Itahara, Y., Nakajima, K., Yoshizawa-Kumagaye, K., Takei, Y.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Atrial Natriuretic Factor - pharmacology
Cattle
Chickens
Chromatography, Gel
Chromatography, High Pressure Liquid
Diuresis - drug effects
Dogs
Female
Fishes
Heart Ventricles
Humans
Male
Mass Spectrometry
Molecular Sequence Data
Myocardium - chemistry
Natriuretic Peptide, Brain
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - isolation & purification
Nerve Tissue Proteins - pharmacology
Rana catesbeiana
Rats
Rats, Sprague-Dawley
Sequence Homology, Amino Acid
Species Specificity
Swine
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Summary:A new natriuretic peptide with 27 amino acid residues has been isolated from cardiac ventricles of the bullfrog,Rana catesbeiana.Since this ventricular peptide had high sequence identity to B-type (brain) natriuretic peptide (BNP), especially to chicken BNP (74%), we named it bullfrog BNP. Thus, semi-aquatic amphibians have tetrapod-type BNP, but do not seem to have fish-type ventricular natriuretic peptide (VNP) in their ventricles. Compared with other known BNPs, the C-terminus of bullfrog BNP was elongated by two amino acid residues and was not amidated. Bullfrog BNP dose-dependently decreased arterial blood pressure in the bullfrog with a potency twofold greater than that of human ANP. Bullfrog BNP also exhibited vasodepressor, natriuretic and diuretic activities in the rat, but it was 1/3, 1/7, and 1/17 as potent as human ANP in this mammalian species.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0743