The kinetics of expression of individual herpes simplex virus type 1 transcripts

We have used specific single-stranded DNA probes cloned in M13 to analyze the kinetics of expression of 20 individual herpes simplex virus type 1 (HSV-1) transcripts, most of known function. The three alpha transcripts studied all differ in the details of their shut-off. There are at least three gro...

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Bibliographic Details
Published in:Virus genes 1987-11, Vol.1 (1), p.49-60
Main Authors: Zhang, Y F, Wagner, E K
Format: Article
Language:English
Subjects:
Animals
Cloning, Molecular
DNA Probes - isolation & purification
Gene Expression Regulation
Kinetics
Nucleic Acid Hybridization
Rabbits
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
RNA, Viral - analysis
RNA, Viral - biosynthesis
Simplexvirus - genetics
Skin - microbiology
Transcription, Genetic
Tritium
Uridine - metabolism
Virus Diseases - genetics
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Summary:We have used specific single-stranded DNA probes cloned in M13 to analyze the kinetics of expression of 20 individual herpes simplex virus type 1 (HSV-1) transcripts, most of known function. The three alpha transcripts studied all differ in the details of their shut-off. There are at least three groups of beta or early transcripts which differ both in the time of their maximum rate of synthesis and in the time of their shut off. Similarly late (beta gamma and gamma) transcripts form a complex group all of which share the fact that their rates of synthesis reach a maximum at a time when beta transcripts are being shut off.
ISSN:0920-8569
DOI:10.1007/bf00125685