Loading…
Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C
Institute for Clinical Microbiology and Immunology, Frohbergstrasse 3, CH-9001 St Gallen, Switzerland The RNA genome of hepatitis A virus (HAV) encodes a giant polyprotein that is putatively cleaved proteolytically into four structural and seven non-structural proteins. So far, most of the proposed...
Saved in:
| Published in: | Journal of general virology 1996-02, Vol.77 (2), p.247-255 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c405t-23ef6eab6897ce3e814628e639d8ce2436d60f7e82707b5cbf08b9c14c7413e83 |
|---|---|
| cites | |
| container_end_page | 255 |
| container_issue | 2 |
| container_start_page | 247 |
| container_title | Journal of general virology |
| container_volume | 77 |
| creator | Gosert, Rainer Cassinotti, Pascal Siegl, Gunter Weitz, Manfred |
| description | Institute for Clinical Microbiology and Immunology, Frohbergstrasse 3, CH-9001 St Gallen, Switzerland
The RNA genome of hepatitis A virus (HAV) encodes a giant polyprotein that is putatively cleaved proteolytically into four structural and seven non-structural proteins. So far, most of the proposed non-structural proteins and their respective cleavage sites have not been identified. A vaccinia virus recombinant (vRGORF) containing the complete HAV ORF under the control of the bacteriophage T7 promoter was used to express HAV in recombinant animal cells (BT7-H) that constitutively expressed T7 DNA-dependent RNA polymerase. A HAV-specific 27.5 kDa expression product was identified as peptide 2B. The 27.5 kDa 2B antigen was also found in HAV-infected MRC-5 cells. The N-terminal amino acid residues of the new peptide 2B are Ala-Lys-Ile-Ser-Leu-Phe and polyprotein cleavage between 2A and 2B occurred at amino acids 836837 (Gln-Ala). Furthermore, heterologous expression in the same system of regions P1P2 and of the protease 3C (3C pro ) gene, showed that P1P2 polyprotein is not cleaved autocatalytically but by 3C pro . Hence, 3C pro is effective in cleaving the polyprotein 2A2B junction.
* Author for correspondence. Fax +41 71 258906. e-mail weitz@comp.bioz.unibas.ch
Received 14 June 1995;
accepted 18 September 1995. |
| doi_str_mv | 10.1099/0022-1317-77-2-247 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78067179</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78067179</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-23ef6eab6897ce3e814628e639d8ce2436d60f7e82707b5cbf08b9c14c7413e83</originalsourceid><addsrcrecordid>eNqFkUtPLCEQhYnReMfHHzC5CStdoUDTQC914isxcaNrQtPVDqYfI9Aa_720M5mtK0jOd06lTiF0xuglo1V1RSnnhBVMEaUIJ1yoPbRgQpaEZ3kfLXbAP3QU4zulTIhSHaJDLbniXC9Q99jAkHzrnU1-HPDY4hWs8z_5iK_xpw9TxMM4kJjC5NIUbIfXYUzgB8xvsB0a7FPEATqwEXD9jdMKcG_fx7A1_9KzVixP0EFruwin2_cYvd7dviwfyNPz_ePy-ok4QctEeAGtBFtLXSkHBei8Etcgi6rRDrgoZCNpq0BzRVVdurqluq4cE04JlvHiGJ1vcvPsjwliMr2PDrrODjBO0ShNpWKq-hNkpZRSizmRb0AXxhgDtGYdfG_Dt2HUzLcwc9VmrtooZbjJt8im_9v0qe6h2Vm25Wf9YqOv_NvqywcwbzD0Po-o_Whye7ukH7g3kpM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15666848</pqid></control><display><type>article</type><title>Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C</title><source>Freely Accessible Science Journals - check A-Z of ejournals</source><creator>Gosert, Rainer ; Cassinotti, Pascal ; Siegl, Gunter ; Weitz, Manfred</creator><creatorcontrib>Gosert, Rainer ; Cassinotti, Pascal ; Siegl, Gunter ; Weitz, Manfred</creatorcontrib><description>Institute for Clinical Microbiology and Immunology, Frohbergstrasse 3, CH-9001 St Gallen, Switzerland
The RNA genome of hepatitis A virus (HAV) encodes a giant polyprotein that is putatively cleaved proteolytically into four structural and seven non-structural proteins. So far, most of the proposed non-structural proteins and their respective cleavage sites have not been identified. A vaccinia virus recombinant (vRGORF) containing the complete HAV ORF under the control of the bacteriophage T7 promoter was used to express HAV in recombinant animal cells (BT7-H) that constitutively expressed T7 DNA-dependent RNA polymerase. A HAV-specific 27.5 kDa expression product was identified as peptide 2B. The 27.5 kDa 2B antigen was also found in HAV-infected MRC-5 cells. The N-terminal amino acid residues of the new peptide 2B are Ala-Lys-Ile-Ser-Leu-Phe and polyprotein cleavage between 2A and 2B occurred at amino acids 836837 (Gln-Ala). Furthermore, heterologous expression in the same system of regions P1P2 and of the protease 3C (3C pro ) gene, showed that P1P2 polyprotein is not cleaved autocatalytically but by 3C pro . Hence, 3C pro is effective in cleaving the polyprotein 2A2B junction.
* Author for correspondence. Fax +41 71 258906. e-mail weitz@comp.bioz.unibas.ch
Received 14 June 1995;
accepted 18 September 1995.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-77-2-247</identifier><identifier>PMID: 8627228</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>3C Viral Proteases ; Amino Acid Sequence ; Animals ; Base Sequence ; Cysteine Endopeptidases - pharmacology ; hepatitis A virus ; Hepatovirus - chemistry ; Immune Sera - immunology ; Molecular Sequence Data ; Rabbits ; Vaccinia virus - genetics ; Viral Nonstructural Proteins - analysis ; Viral Nonstructural Proteins - chemistry ; Viral Nonstructural Proteins - immunology ; Viral Proteins</subject><ispartof>Journal of general virology, 1996-02, Vol.77 (2), p.247-255</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-23ef6eab6897ce3e814628e639d8ce2436d60f7e82707b5cbf08b9c14c7413e83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8627228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gosert, Rainer</creatorcontrib><creatorcontrib>Cassinotti, Pascal</creatorcontrib><creatorcontrib>Siegl, Gunter</creatorcontrib><creatorcontrib>Weitz, Manfred</creatorcontrib><title>Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Institute for Clinical Microbiology and Immunology, Frohbergstrasse 3, CH-9001 St Gallen, Switzerland
The RNA genome of hepatitis A virus (HAV) encodes a giant polyprotein that is putatively cleaved proteolytically into four structural and seven non-structural proteins. So far, most of the proposed non-structural proteins and their respective cleavage sites have not been identified. A vaccinia virus recombinant (vRGORF) containing the complete HAV ORF under the control of the bacteriophage T7 promoter was used to express HAV in recombinant animal cells (BT7-H) that constitutively expressed T7 DNA-dependent RNA polymerase. A HAV-specific 27.5 kDa expression product was identified as peptide 2B. The 27.5 kDa 2B antigen was also found in HAV-infected MRC-5 cells. The N-terminal amino acid residues of the new peptide 2B are Ala-Lys-Ile-Ser-Leu-Phe and polyprotein cleavage between 2A and 2B occurred at amino acids 836837 (Gln-Ala). Furthermore, heterologous expression in the same system of regions P1P2 and of the protease 3C (3C pro ) gene, showed that P1P2 polyprotein is not cleaved autocatalytically but by 3C pro . Hence, 3C pro is effective in cleaving the polyprotein 2A2B junction.
* Author for correspondence. Fax +41 71 258906. e-mail weitz@comp.bioz.unibas.ch
Received 14 June 1995;
accepted 18 September 1995.</description><subject>3C Viral Proteases</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cysteine Endopeptidases - pharmacology</subject><subject>hepatitis A virus</subject><subject>Hepatovirus - chemistry</subject><subject>Immune Sera - immunology</subject><subject>Molecular Sequence Data</subject><subject>Rabbits</subject><subject>Vaccinia virus - genetics</subject><subject>Viral Nonstructural Proteins - analysis</subject><subject>Viral Nonstructural Proteins - chemistry</subject><subject>Viral Nonstructural Proteins - immunology</subject><subject>Viral Proteins</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkUtPLCEQhYnReMfHHzC5CStdoUDTQC914isxcaNrQtPVDqYfI9Aa_720M5mtK0jOd06lTiF0xuglo1V1RSnnhBVMEaUIJ1yoPbRgQpaEZ3kfLXbAP3QU4zulTIhSHaJDLbniXC9Q99jAkHzrnU1-HPDY4hWs8z_5iK_xpw9TxMM4kJjC5NIUbIfXYUzgB8xvsB0a7FPEATqwEXD9jdMKcG_fx7A1_9KzVixP0EFruwin2_cYvd7dviwfyNPz_ePy-ok4QctEeAGtBFtLXSkHBei8Etcgi6rRDrgoZCNpq0BzRVVdurqluq4cE04JlvHiGJ1vcvPsjwliMr2PDrrODjBO0ShNpWKq-hNkpZRSizmRb0AXxhgDtGYdfG_Dt2HUzLcwc9VmrtooZbjJt8im_9v0qe6h2Vm25Wf9YqOv_NvqywcwbzD0Po-o_Whye7ukH7g3kpM</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Gosert, Rainer</creator><creator>Cassinotti, Pascal</creator><creator>Siegl, Gunter</creator><creator>Weitz, Manfred</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C</title><author>Gosert, Rainer ; Cassinotti, Pascal ; Siegl, Gunter ; Weitz, Manfred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-23ef6eab6897ce3e814628e639d8ce2436d60f7e82707b5cbf08b9c14c7413e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>3C Viral Proteases</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cysteine Endopeptidases - pharmacology</topic><topic>hepatitis A virus</topic><topic>Hepatovirus - chemistry</topic><topic>Immune Sera - immunology</topic><topic>Molecular Sequence Data</topic><topic>Rabbits</topic><topic>Vaccinia virus - genetics</topic><topic>Viral Nonstructural Proteins - analysis</topic><topic>Viral Nonstructural Proteins - chemistry</topic><topic>Viral Nonstructural Proteins - immunology</topic><topic>Viral Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gosert, Rainer</creatorcontrib><creatorcontrib>Cassinotti, Pascal</creatorcontrib><creatorcontrib>Siegl, Gunter</creatorcontrib><creatorcontrib>Weitz, Manfred</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gosert, Rainer</au><au>Cassinotti, Pascal</au><au>Siegl, Gunter</au><au>Weitz, Manfred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>77</volume><issue>2</issue><spage>247</spage><epage>255</epage><pages>247-255</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>Institute for Clinical Microbiology and Immunology, Frohbergstrasse 3, CH-9001 St Gallen, Switzerland
The RNA genome of hepatitis A virus (HAV) encodes a giant polyprotein that is putatively cleaved proteolytically into four structural and seven non-structural proteins. So far, most of the proposed non-structural proteins and their respective cleavage sites have not been identified. A vaccinia virus recombinant (vRGORF) containing the complete HAV ORF under the control of the bacteriophage T7 promoter was used to express HAV in recombinant animal cells (BT7-H) that constitutively expressed T7 DNA-dependent RNA polymerase. A HAV-specific 27.5 kDa expression product was identified as peptide 2B. The 27.5 kDa 2B antigen was also found in HAV-infected MRC-5 cells. The N-terminal amino acid residues of the new peptide 2B are Ala-Lys-Ile-Ser-Leu-Phe and polyprotein cleavage between 2A and 2B occurred at amino acids 836837 (Gln-Ala). Furthermore, heterologous expression in the same system of regions P1P2 and of the protease 3C (3C pro ) gene, showed that P1P2 polyprotein is not cleaved autocatalytically but by 3C pro . Hence, 3C pro is effective in cleaving the polyprotein 2A2B junction.
* Author for correspondence. Fax +41 71 258906. e-mail weitz@comp.bioz.unibas.ch
Received 14 June 1995;
accepted 18 September 1995.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>8627228</pmid><doi>10.1099/0022-1317-77-2-247</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1996-02, Vol.77 (2), p.247-255 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78067179 |
| source | Freely Accessible Science Journals - check A-Z of ejournals |
| subjects | 3C Viral Proteases Amino Acid Sequence Animals Base Sequence Cysteine Endopeptidases - pharmacology hepatitis A virus Hepatovirus - chemistry Immune Sera - immunology Molecular Sequence Data Rabbits Vaccinia virus - genetics Viral Nonstructural Proteins - analysis Viral Nonstructural Proteins - chemistry Viral Nonstructural Proteins - immunology Viral Proteins |
| title | Identification of hepatitis A virus non-structural protein 2B and its release by the major virus protease 3C |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T22%3A08%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20hepatitis%20A%20virus%20non-structural%20protein%202B%20and%20its%20release%20by%20the%20major%20virus%20protease%203C&rft.jtitle=Journal%20of%20general%20virology&rft.au=Gosert,%20Rainer&rft.date=1996-02-01&rft.volume=77&rft.issue=2&rft.spage=247&rft.epage=255&rft.pages=247-255&rft.issn=0022-1317&rft.eissn=1465-2099&rft_id=info:doi/10.1099/0022-1317-77-2-247&rft_dat=%3Cproquest_cross%3E78067179%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c405t-23ef6eab6897ce3e814628e639d8ce2436d60f7e82707b5cbf08b9c14c7413e83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15666848&rft_id=info:pmid/8627228&rfr_iscdi=true |