Purification and reconstitution of the calcium release channel from skeletal muscle

The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junct...

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Bibliographic Details
Published in:Nature (London) 1988-01, Vol.331 (6154), p.315-319
Main Authors: Anthony Lai, F, Erickson, Harold P, Rousseau, Eric, Liu, Qi-Yi, Meissner, Gerhard
Format: Article
Language:English
Subjects:
Anatomy & physiology
Animals
Biochemistry
Biological and medical sciences
Calcium
Calcium - metabolism
Cell physiology
Fundamental and applied biological sciences. Psychology
Ion Channels - analysis
Ion Channels - metabolism
Ion Channels - ultrastructure
Liposomes
Medical research
Membrane and intracellular transports
Membrane Potentials
Molecular and cellular biology
Rabbits
Receptors, Cholinergic - isolation & purification
Receptors, Cholinergic - metabolism
Ryanodine Receptor Calcium Release Channel
Sarcoplasmic Reticulum - analysis
Sarcoplasmic Reticulum - ultrastructure
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Summary:The calcium release channel from rabbit muscle sarcoplasmic reticulum (SR) has been purified and reconstituted as a functional unit in lipid bilayers. Electron microscopy reveals the four-leaf clover structure previously described for the 'feet' that span the transverse tubule (T)-SR junction. Ca2+ release from the SR induced by T-system depolarization during excitation-contraction coupling in muscle may thus be effected through a direct association of the T-system with SR Ca2+-release channels.
ISSN:0028-0836
1476-4687
DOI:10.1038/331315a0