Ultrafiltration discrepancies in recovery of myoglobin from urine

We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the origina...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1996-06, Vol.42 (6), p.965-969
Main Authors: Loun, B, Copeland, KR, Sedor, FA
Format: Article
Language:English
Subjects:
Biological and medical sciences
False Negative Reactions
Humans
Hydrogen-Ion Concentration
Investigative techniques, diagnostic techniques (general aspects)
Kidney Diseases - urine
Medical sciences
Microchemistry
Myoglobinuria - urine
Osteoarticular system. Muscles
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reagent Strips
Reproducibility of Results
Ultrafiltration
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Summary:We investigated the efficiency, accuracy, and reliability of the ultrafiltration/dipstick methodology commonly used to diagnose myoglobinuria. Twenty-five myoglobin-containing urine specimens were filtered by centrifugation for 15 min at 1500g through a Centricon-30 membrane filter. Both the original specimen and filtrate were assayed for myoglobin. The amount of myoglobin recovered subsequent to filtration varied from
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/42.6.965