80% of muscarinic receptors expressed by the NB-OK 1 human neuroblastoma cell line show high affinity for pirenzepine and are comparable to rat hippocampus M1 receptors

The NB-OK 1 human neuroblastoma cell line expressed muscarinic cholinergic receptors that could be labeled with N-[ 3H]methylscopolamine (a nonselective antagonist). 80% of these receptors showed high affinity for pirenzepine, i.e. belonged to the M1 subtype found in neuronal tissues. Their binding...

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Bibliographic Details
Published in:FEBS letters 1988-01, Vol.226 (2), p.287-290
Main Authors: Waelbroeck, Magali, Camus, Jean, Tastenoy, Michèle, Christophe, Jean
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Cell receptors
Cell structures and functions
Fundamental and applied biological sciences. Psychology
Hippocampus - metabolism
Humans
Kinetics
Molecular and cellular biology
Muscarinic receptor
NB-OK cell, Human, Rat pancreas, Rat hippocampus
Neuroblastoma
Neuroblastoma - metabolism
Pirenzepine - metabolism
Rats
Receptor binding assay
Receptor subtype
Receptors, Muscarinic - metabolism
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Summary:The NB-OK 1 human neuroblastoma cell line expressed muscarinic cholinergic receptors that could be labeled with N-[ 3H]methylscopolamine (a nonselective antagonist). 80% of these receptors showed high affinity for pirenzepine, i.e. belonged to the M1 subtype found in neuronal tissues. Their binding properties were identical to those of rat hippocampus M1 receptors, and differed from those of rat pancreas and heart muscarinic receptors. The remaining (20%) muscarinic receptors showed low affinity for pirenzepine and AF-DX 116, being therefore of an M2β (or B) subtype, and were similar to rat pancreatic receptors.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)81441-8