Heat-labile uracil-DNA glycosylase: purification and characterization

A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) wit...

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Bibliographic Details
Published in:FEBS letters 1996-06, Vol.388 (1), p.1-4
Main Authors: Sobek, H., Schmidt, M., Frey, B., Kaluza, K.
Format: Article
Language:English
Subjects:
Amplification
Bacteria - enzymology
Base Sequence
bovine serum albumin
BSA
Carryover prevention
DNA Glycosylases
Enzyme Stability
Escherichia coli
Half-Life
Hot Temperature
Marine
Molecular Sequence Data
Molecular Weight
N-Glycosyl Hydrolases - chemistry
N-Glycosyl Hydrolases - isolation & purification
N-Glycosyl Hydrolases - metabolism
PCR
UNG
Uracil-DNA Glycosidase
Uracil-DNA glycosylase
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Description
Summary:A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23 400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40°C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a slower reactivation rate than the usually applied UNG from Escherichia coli.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00444-9