Ionic conductivity, transference numbers, composition and mobility of ions in cross-linked lysozyme crystals

Micromethods for measurements of electric conductivity, transference numbers and concentrations of inorganic ions within immobilized protein crystals have been developed and applied to study tetragonal lysozyme crystals cross-linked with glutaraldehyde. Donnan equilibria and mobilities of ions in th...

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Bibliographic Details
Published in:Biophysical chemistry 1996-05, Vol.60 (1), p.1-16
Main Authors: Morozova, T.Ya, Kachalova, G.S., Lanina, N.F., Evtodienko, V.U., Botin, A.S., Shlyapnikova, E.A., Morozov, V.N.
Format: Article
Language:English
Subjects:
Chemical Phenomena
Chemistry, Physical
Cross-Linking Reagents - chemistry
Crystallization
Electric conductance
Electric Conductivity
Enzyme Activation
Glutaral - chemistry
Hydrogen-Ion Concentration
Ion Channels - chemistry
Ionic composition
Ionic mobility
Ions
Lysozyme crystals
Muramidase - chemistry
Osmolar Concentration
pH titration curve
Sodium Chloride - chemistry
Transference numbers
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Summary:Micromethods for measurements of electric conductivity, transference numbers and concentrations of inorganic ions within immobilized protein crystals have been developed and applied to study tetragonal lysozyme crystals cross-linked with glutaraldehyde. Donnan equilibria and mobilities of ions in this crystal were calculated using the data of these methods and the data of crystal pH titration. Taken together these results characterize the lysozyme crystal as an ion exchanger whose electrical properties and ion composition differ greatly from those of the external solution. Although anions transfer most of the current in the crystals, anion mobility is considerably lower than that of cations. Mobility of all ions in the crystal is considerably lower than in solution (3.5–50 times for cations and 120–330 times for anions) and depends on steric restrictions and charges of both ions and lysozyme molecules. Similar features in behavior of crystalline and biological channels are discussed.
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(96)00007-5