DNA inclusions within autolytic cytoplasmic vacuoles of hemopoietic stem cell line FDCP-Mix

FDCP-Mix, a pluripotent routine hemopoietic stem cell line undergoes internucleosomal cleavage of DNA when induced to apoptosis either by drugs or by withdrawal of growth factor (IL-3), and also displays a pattern of nuclear morphology that is typical for apoptosis. However, increased autolytic acti...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1996-06, Vol.44 (6), p.549-558
Main Authors: Reipert, S, Hickman, JA, Allen, TD
Format: Article
Language:English
Subjects:
Animals
Autolysis
Cell Line
Deoxyribonuclease I - metabolism
DNA - metabolism
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - metabolism
Hematopoietic Stem Cells - ultrastructure
Inclusion Bodies - metabolism
Mice
Mitochondria - metabolism
Vacuoles - metabolism
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Summary:FDCP-Mix, a pluripotent routine hemopoietic stem cell line undergoes internucleosomal cleavage of DNA when induced to apoptosis either by drugs or by withdrawal of growth factor (IL-3), and also displays a pattern of nuclear morphology that is typical for apoptosis. However, increased autolytic activity in the cytoplasm precedes the nuclear changes. For etoposide-treated FDCP-Mix cells, mitochondria were identified as a target for autolytic digestion in large autolytic vacuoles, but during this period an increase in the number of mitochondria was observed. The autolytic vacuoles displayed variations in their content. Large, electron-dense inclusions resembling "condensed chromatin" could regularly be found in FDCP-Mix cells treated with low concentrations of etoposide (
ISSN:0022-1554
1551-5044
DOI:10.1177/44.6.8666740