Comparison of the genomic short regions of a vaccine strain (SA-2) and a virulent strain (CSW-1) of infectious laryngotracheitis virus (Gallid herpesvirus 1)

• Published in: Avian diseases 1996-01, Vol.40 (1), p.130-139
• Main Authors: Trist, H.M. (CSIRO, Parkville, Australia.), Tyack, S.G, Johnson, M.A, Prideaux, C.T, Sheppard, M
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Chickens; Chickens - virology; DIFERENCIAS BIOLOGICAS; DIFFERENCE BIOLOGIQUE; DNA; DNA, Viral - genetics; Enzymes; Gels; GENETICA; GENETIQUE; Genomes; Herpesviridae; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus 1, Gallid - classification; Herpesvirus 1, Gallid - genetics; Molecular Sequence Data; Plasmids; Poultry Diseases - virology; Restriction Mapping; SECUENCIA NUCLEICA; Sequence Analysis, DNA; SEQUENCE NUCLEIQUE; Species Specificity; Terminal repeat sequences; VACCIN; Vaccination; VACUNA; VARIACION GENETICA; VARIATION GENETIQUE; VIRUS DE LA LARINGOTRAQUEITIS AVIAR; VIRUS DE LA LTI; Viruses
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1592382

Restriction enzyme linkage maps were produced for the genomic short region of the virulent infectious laryngotracheitis virus (CSW-1 strain). After comparison with the equivalent restriction enzyme linkage maps for the infectious laryngotracheitis virus SA-2 strain (a vaccine strain), it was determined that the maps for the short regions of the two strains were identical, apart from a single section in each of the inverted terminal repeats. Each inverted terminal repeat of the SA-2 strain was discovered to contain 467 base pairs more DNA than the CSW-1 strain's inverted terminal repeats. This extra DNA was more precisely mapped entirely within the EcoRI fragments D and d of SA-2, which were found to form part of the SmaI fragments U and P of SA-2 and Q and b of SA-2 and to contain one SmaI restriction enzyme site