Epithelial tumour cells in bone marrow of patients with pancreatic carcinoma detected by immunocytochemical staining

In the present study, epithelial cells in the bone marrow of 42 patients with pancreatic carcinoma were identified immunocytochemically with monoclonal antibodies (MAbs) CK2, KL1 and A45-B/B3 directed to epithelial cytokeratins (CK), using the alkaline phosphatase anti-alkaline phosphatase method. T...

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Bibliographic Details
Published in:European journal of cancer (1990) 1996-02, Vol.32 (2), p.363-365
Main Authors: Thorban, S., Roder, J.D., Pantel, K., Siewert, J.R.
Format: Article
Language:English
Subjects:
Adenocarcinoma - diagnosis
Adenocarcinoma - metabolism
Adenocarcinoma - secondary
Antibodies, Monoclonal
Bone Marrow Neoplasms - diagnosis
Bone Marrow Neoplasms - metabolism
Bone Marrow Neoplasms - secondary
cytokeratins
Follow-Up Studies
Humans
immunocytochemistry
Immunoenzyme Techniques
Keratins - metabolism
micrometastasis
monoclonal antibodies
Neoplasm Proteins - metabolism
Neoplasm Recurrence, Local
pancreatic carcinoma
Pancreatic Neoplasms - pathology
Prospective Studies
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Summary:In the present study, epithelial cells in the bone marrow of 42 patients with pancreatic carcinoma were identified immunocytochemically with monoclonal antibodies (MAbs) CK2, KL1 and A45-B/B3 directed to epithelial cytokeratins (CK), using the alkaline phosphatase anti-alkaline phosphatase method. The specificity of the MAbs was demonstrated by negative staining of marrow from 25 noncarcinoma age-matched control patients. Analysis of bone marrow aspirates from cancer patients revealed CK-positive cells in 14 (58.3%) of 24 cancer patients treated with curative intent and 10 (55.6%) of 18 patients with extended disease. After a median follow-up of 15.6 months (range 3–31 months), 5 (35.7%) out of 14 patients who underwent complete surgical resection but had tumour cells in bone marrow presented with distant metastasis and 6 (42.9%) with local relapse as compared to none of 10 corresponding patients without such cells ( P < 0.05). The described technique may help to identify patients with pancreatic cancer and potential high risk of early metastatic relapse. The results promise to be of important assistance in determining prognosis and consequences in therapy of early stage pancreatic cancer.
ISSN:0959-8049
1879-0852
DOI:10.1016/0959-8049(95)00586-2