BstF5I, an unusual isoschizomer of FokI

BstF5I, a new restriction endonuclease (ENase) from Bacillus stearothermophilus F5, has been discovered. This enzyme recognizes 5′-GGATG-3′ and cleaves DNA, generating a 2-base 3′ extension: 5′ - G G A T G NN∨ - 3′ 3′ - C C T A C ∧ NN - 5′ BstF5I is an isoschizomer of FokI and seems to be evolutiona...

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Bibliographic Details
Published in:Gene 1996-06, Vol.172 (1), p.49-51
Main Authors: Abdurashitov, M.A., Kileva, E.V., Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh
Format: Article
Language:English
Subjects:
Bacillus stearothermophilus
Base Sequence
cleavage points
Deoxyribonucleases, Type II Site-Specific - metabolism
Geobacillus stearothermophilus - enzymology
Molecular Sequence Data
recognition sequence
Restriction endonuclease
Substrate Specificity
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Summary:BstF5I, a new restriction endonuclease (ENase) from Bacillus stearothermophilus F5, has been discovered. This enzyme recognizes 5′-GGATG-3′ and cleaves DNA, generating a 2-base 3′ extension: 5′ - G G A T G NN∨ - 3′ 3′ - C C T A C ∧ NN - 5′ BstF5I is an isoschizomer of FokI and seems to be evolutionarily close to other nonpalindromic-recognizing ENases from thermophilic bacilli.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(96)85083-9