Protein Tyrosine Kinase Inhibitors Promote Amylase Secretion and Inhibit Ornithine Decarboxylase Induction in Sialagogue-Stimulated Rat Parotid Explants

Three sialagogues, isoproterenol (IPR), carbachol, and methoxamine, caused induction of ornithine decarboxylase (ODC) in cultured rat parotid explants. All the protein tyrosine kinase inhibitors tested suppressed this ODC induction but enhanced sialagogue-dependent amylase secretion. Sodium orthovan...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-06, Vol.223 (1), p.170-174
Main Authors: Kinoshita, Fumiyo, Ueno, Akemichi, Miwa, Yoshihiro, Nishino, Mizuho, Inoue, Hideo
Format: Article
Language:English
Subjects:
Amylases - secretion
Animals
Benzoquinones
Bucladesine - pharmacology
Carbachol - pharmacology
Chamomile
Cholecystokinin - pharmacology
Cinnamates - pharmacology
Cyclic AMP - metabolism
Enzyme Induction - drug effects
Enzyme Inhibitors - pharmacology
Flavonoids - pharmacology
Genistein
Isoflavones - pharmacology
Isoproterenol - pharmacology
Lactams, Macrocyclic
Male
Methoxamine - pharmacology
Oils, Volatile - pharmacology
Organ Culture Techniques
Ornithine Decarboxylase - biosynthesis
Parotid Gland - drug effects
Parotid Gland - enzymology
Phenols - pharmacology
Plants, Medicinal
Protein-Tyrosine Kinases - antagonists & inhibitors
Quinones - pharmacology
Rats
Rats, Wistar
Rifabutin - analogs & derivatives
Sialic Acids - secretion
Vanadates - pharmacology
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Summary:Three sialagogues, isoproterenol (IPR), carbachol, and methoxamine, caused induction of ornithine decarboxylase (ODC) in cultured rat parotid explants. All the protein tyrosine kinase inhibitors tested suppressed this ODC induction but enhanced sialagogue-dependent amylase secretion. Sodium orthovanadate showed the reverse effects as the kinase inhibitors. Immunoblot analysis with anti-phosphotyrosine antibody revealed that herbimycin A depresses IPR-stimulated tyrosine phosphorylation of parotid proteins. Herbimycin A did not affect the IPR- or dibutyryl cAMP-induced surge of the parotid cAMP level but inhibited these agonist-dependent ODC inductions. These results suggest that sialagogue-induced ODC induction and amylase secretion are mediated by different signal transduction pathways and that protein tyrosine kinase participates in IPR-dependent ODC induction and amylase secretion in the process subsequent to the cAMP surge.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0864