Ontogenies of messenger RNA encoding tissue inhibitor of metalloproteinases 1 and 2 within bovine periovulatory follicles and luteal tissue

• Published in: Domestic animal endocrinology 1996-03, Vol.13 (2), p.151-160
• Main Authors: Smith, G.W., Juengel, J.L., Mclntush, E.W., Youngquist, R.S., Garverick, H.A., Smith, M.F.
• Format: Article
• Language: English
• Subjects: Animals; ARN MENSAJERO; ARN MESSAGER; Blotting, Northern; Cattle; CICLO ESTRAL; CORPS JAUNE; CORPUS HEMORRHAGICUM; CORPUS LUTEUM; Corpus Luteum - metabolism; CUERPO LUTEO; CYCLE OESTRAL; ENZYME INHIBITORS; EXPRESION GENICA; EXPRESSION DES GENES; Female; Follicle Stimulating Hormone - blood; GENE EXPRESSION; GENISSE; Glycoproteins - genetics; GNRH; GONADOLIBERINE; GONADOTROPIN RELEASING HORMONE; Gonadotropin-Releasing Hormone - pharmacology; GRAAFIAN FOLLICLES; HEIFERS; HORMONA LIBERADORA DE GONADOTROPINA; INHIBIDORES DE ENZIMAS; INHIBITEUR D'ENZYME; LUTEAL PHASE; Luteinizing Hormone - blood; LUTEOLYSIS; MESSENGER RNA; NOVILLA; OESTROUS CYCLE; OVAIRE; Ovarian Follicle - metabolism; Ovariectomy; OVARIES; OVARIOS; OVULACION; OVULATION; PREOVULATORY PERIOD; Progesterone - blood; PROSTAGLANDINAS; PROSTAGLANDINE; PROSTAGLANDINS; PROTEASAS; PROTEASE; PROTEASES; Proteins - genetics; RNA, Messenger - metabolism; Tissue Inhibitor of Metalloproteinase-2; Tissue Inhibitor of Metalloproteinases
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/0739-7240(95)00065-8

Tissue inhibitors of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) are important regulators of extracellular matrix remodeling and also possess growth factor activity. The objective of these studies was to characterize TIMP-1 and TIMP-2 mRNA expression by bovine periovulatory follicles/corpora hemorrhagica (Experiment 1) and luteal tissue (Experiment 2). In Experiment 1, beef heifers ( n = 27) were ovariectomized at −16 ( n = 6), 0 ( n = 5), 8 ( n = 3), 16 ( n = 4), 24 ( n = 4), or 48 ( n = 5) hr relative to a gonadotropin-releasing hormone induced gonadotropin surge (40 hr after prostaglandin F 2α-induced luteolysis). Total cellular RNA was isolated from the large steroidogenically active follicle or corpus hemorrhagicum obtained from each animal, and the expression of TIMP-1 and TIMP-2 mRNA was subsequently examined by northern and dot blot analysis. The expression of TIMP-1 or TIMP-2 mRNA did not differ in preovulatory follicles collected at −16 vs. 0 hr. Concentrations of both TIMP-1 and TIMP-2 mRNA (picograms per microgram of tissue DNA) were increased ( P < 0.05) at 8 hr postgonadotropin surge, had declined to presurge levels by 24 hr ( P < 0.05), and were increased ( P < 0.05) in corpora hemorrhagica collected at 48 hr after a gonadotropin surge. In Experiment 2, corpora lutea were collected from beef heifers on Days 4, 10, 15 ( n = 4 each), or 19 ( n = 3) postestrus (Day 0 = estrus). Concentrations of TIMP-1 mRNA (picograms per microgram of tissue DNA) were greater in corpora lutea collected on Day 4 ( P < 0.05) vs. Day 10, 15, or 19. Concentrations of TIMP-2 mRNA increased ( P < 0.05) from Day 4 to 15 and decreased ( P < 0.05) by Day 19. We conclude that: 1) during the periovulatory period, the ontogenies of TIMP-1 and TIMP-2 mRNA expression are similar, whereas 2) during luteal phase, TIMP-1 mRNA expression is maximal during the early luteal phase, whereas concentrations of TIMP-2 mRNA peak during the midluteal phase. TIMP-1 and TIMP-2 may play important roles in the regulation of extracellular matrix remodeling during the periovulatory period and the subsequent luteal phase.