Molecular Analysis of Decreased Interleukin-12 Production in Persons Infected with Human Immunodeficiency Virus

Human immunodeficiency virus (HIV) disease is associated with loss of type 1 responses, including interleukin (IL)-12 production. The dramatic drop in p70 production seen at early stages of disease was found not to be associated with a similarly decreased p40 mRNA expression. p35 mRNA expression was...

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Bibliographic Details
Published in:The Journal of infectious diseases 1996-07, Vol.174 (1), p.46-53
Main Authors: Chougnet, Claire, Wynn, Thomas A., Clerici, Mario, Landay, Alan L., Kessler, Harold A., Rusnak, Janice, Melcher, Gregory P., Sher, Alan, Shearer, Gene M.
Format: Article
Language:English
Subjects:
AIDS
AIDS/HIV
Antigen presenting cells
Base Sequence
Biological and medical sciences
Blood
Cytokines
Gene expression regulation
Gene Expression Regulation, Viral
HIV
HIV Infections - metabolism
human immunodeficiency virus
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Interleukin-10 - biosynthesis
Interleukin-10 - genetics
Interleukin-10 - physiology
Interleukin-12 - biosynthesis
Interleukin-12 - genetics
Major Articles
Medical sciences
Messenger RNA
Molecular Sequence Data
Monocytes
Peripheral blood mononuclear cells
RNA, Messenger - blood
T lymphocytes
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Summary:Human immunodeficiency virus (HIV) disease is associated with loss of type 1 responses, including interleukin (IL)-12 production. The dramatic drop in p70 production seen at early stages of disease was found not to be associated with a similarly decreased p40 mRNA expression. p35 mRNA expression was more extensively reduced than p40mRNA expression at these early stages. Monocytes infected in vitro with HIV displayed decreased p35 expression and p70 production, suggesting that such decreased IL-12 expression may contribute to reduced IL-12 production in HIV-positive patients' cells. In addition, treatment of cells with IL-10 increased IL-10 mRNA expression and decreased p40 expression in both HIV-positive and -negative cells, while neutralization of IL-10 increased p40 mRNA levels. These observations, together with the observed hyperproduction of IL-10 in HIV-positive patients, may explain the dysregulation of IL-12 production seen in HIV disease.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/174.1.46