Gene organization in the ada-rplL region of Streptomyces virginiae

• Published in: Gene 1996-05, Vol.171 (1), p.135-136
• Main Authors: Katayama, Midori, Sakai, Yumi, Okamoto, Susumu, Ihara, Fumio, Nihira, Takuya, Yamada, Yasuhiro
• Format: Article
• Language: English
• Subjects: adenosine deaminase; Adenosine Deaminase - genetics; aspartate aminotransferase; Aspartate Aminotransferases - genetics; Bacterial Proteins - genetics; Cloning, Molecular; DNA sequencing; Escherichia coli Proteins; Genes, Bacterial - genetics; Molecular Sequence Data; nusG; Open Reading Frames - genetics; Restriction Mapping; ribosomal protein; Ribosomal Proteins - genetics; rpIKAJL; SEC Translocation Channels; secE; Sequence Homology, Amino Acid; Streptomyces - genetics; Streptomyces virginiae; Transcription Factors
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(96)00067-4

The gene organization of a 7.4-kb region of the Streptomyces virginiae (Sv) chromosome was determined. The predicted open reading frames (ORFs) and their predicted products, in sequence order, were (i) ada, encoding adenosine deaminase [EC 3.5.4.4], ( ii) aat, encoding a protein homologous to aspartate aminotransferase [EC 2.6.1.1], (iii) secE, encoding a protein involved in protein secretion, (iv) vbrA, encoding a NusG-like protein involved in antitermination of transcription as described by Okamoto et al. [J. Biol. Chem. 267 (1992) 1093–1098], and (v) rplKAJL, encoding the large subunits of the ribosomal proteins Lll, L1, L10 and L12. Six of the ORFs (secE-rplL) were oriented in the same direction, but the other two ( ada and aat) had the opposite orientation. The gene organization of the secE-rplL region in Sv was identical to that in Escherichia coli.