Genetic fingerprinting of Theileria parva using a telomeric DNA probe

A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLPs). Two isolate...

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Bibliographic Details
Published in:Parasitology research (1987) 1996, Vol.82 (3), p.264-266
Main Authors: BISHOP, R. P, SPOONER, P. R, SOHANPAL, B. K
Format: Article
Language:English
Subjects:
Animals
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Buffaloes - parasitology
Cattle
DNA Fingerprinting
DNA Probes
DNA, Protozoan
Fundamental and applied biological sciences. Psychology
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Protozoa
Telomere
Theileria parva - genetics
Ticks - parasitology
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Summary:A cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLPs). Two isolates derived from buffalo 7014 by tick feeding on different occasions were also differentiated using the probe. The probe gave comparable results on purified piroplasm or schizont-infected lymphocyte DNA and did not cross-hybridize with uninfected bovine DNA. The telomeric restriction pattern of a cloned T. parva parasite remained identical after four passages through ticks and cattle. The telomeric sequence therefore represents a useful additional tool for analysis of theileriosis epidemiology.
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050108