Identification of differentially expressed messenger RNAs in human melanocytes and melanoma cells

The phenotype of malignant lesions is a reflection of genetic events altering the RNA and protein expression patterns of normal cells. We have investigated RNA expression patterns distinguishing normal melanocytes (FM 902), a primary melanoma cell line (WM 793), and its variant cell line (1205-LU),...

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Published in:Cancer research (Chicago, Ill.) Ill.), 1996-07, Vol.56 (13), p.3112-3117
Main Authors: SIMON, H.-G, RISSE, B, JOST, M, OPPENHEIMER, S, KARI, C, RODECK, U
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cell Division - physiology
Cell Line
Dermatology
DNA, Complementary - genetics
DNA, Neoplasm - genetics
Evaluation Studies as Topic
Gene Amplification
Gene Expression
Humans
Medical sciences
Melanocytes - metabolism
Melanocytes - physiology
Melanoma - genetics
Melanoma - metabolism
Mice
Molecular Sequence Data
RNA, Messenger - genetics
RNA, Messenger - metabolism
Tumors of the skin and soft tissue. Premalignant lesions
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Summary:The phenotype of malignant lesions is a reflection of genetic events altering the RNA and protein expression patterns of normal cells. We have investigated RNA expression patterns distinguishing normal melanocytes (FM 902), a primary melanoma cell line (WM 793), and its variant cell line (1205-LU), selected for metastatic phenotype in athymic mice. Using mRNA differential display, we identified 42 different cDNA PCR products with cell line-specific expression patterns. Direct sequence analysis matched approximately 50% of the cDNA PCR products with gene sequences accessible in DNA databases. Among the known genes, two functionally distinct groups were recognized: (a) genes encoding ribosomal and mitochondrial proteins that were predominantly up-regulated in the malignant cells; and (b) genes encoding modulators of the immune response. Among the immunomodulators, the T-cell antigen MART-1 and the protease inhibitor alpha2-macroglobulin were detected in the melanocyte cell line but not in the tumor cells. By contrast, mRNAs for the complement inhibitor CD59 and the cytokine IL-1beta were found to be overexpressed in the malignant melanoma cells. RNA slot blot hybridization on a larger panel of melanocyte and melanoma cell lines confirmed differential expression of 15 of 42 genes including MART-1, alpha2-macroglobulin, and CD59. This molecular screening approach identified also three partially characterized and three novel sequences with differential expression patterns in normal and malignant melanocytes.
ISSN:0008-5472
1538-7445