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Short-term effects of pure anti-oestrogen ICI 182780 treatment on oestrogen receptor, epidermal growth factor receptor and transforming growth factor-alpha protein expression in human breast cancer
Expression of oestrogen receptor (ER), epidermal growth factor receptor (EGFR) and transforming growth factor-α (TGFα) proteins was assessed by immunocytochemistry on primary breast cancer specimens obtained before and following short-term (7-day) presurgical exposure to pure anti-oestrogen (7α- [9-...
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Published in: | European journal of cancer (1990) 1996-03, Vol.32 (3), p.413-416 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Expression of oestrogen receptor (ER), epidermal growth factor receptor (EGFR) and transforming growth factor-α (TGFα) proteins was assessed by immunocytochemistry on primary breast cancer specimens obtained before and following short-term (7-day) presurgical exposure to pure anti-oestrogen (7α- [9- (4,4,5,5,5-pentafluoropentylsulphinyl) nonyl] estra-1,3,5, (10)-triene-3,17 β-diol, ICI 182780) treatment and compared with no-treatment controls. Paired needle-core and mastectomy samples were obtained from 21 patients. Effects of ICI 182780 (10 −7M) on MCF7 breast cancer cell ER, EGFR and TGFα expression were also examined over 14 days. ER protein was significantly suppressed by ICI 182780 in vivo (P = 0.009) and comparative analysis of short term ICI 182780 effects in vitro, using ER-positive MCF7 cells, gave largely equivalent results. EGFR and TGFα protein levels were unaltered by treatment. ICI 182780 suppresses ER without a concomitant rise in either EGFR or TGFα. |
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ISSN: | 0959-8049 1879-0852 |
DOI: | 10.1016/0959-8049(95)00517-X |