Mouse RSS spacer sequences affect the rate of V(D)J recombination

The diversity of antigen-specific receptors [immunoglobulins (Ig) and T-cell receptors (Tcr)] is generated largely during the assembly of the genetic elements - named V, D, and J - that encode the variable region of both Ig and Tcr. This assembly involves a somatic, cell-type specific, site-specific...

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Bibliographic Details
Published in:Immunogenetics (New York) 1996, Vol.44 (2), p.146-150
Main Authors: Fanning, L, Connor, A, Baetz, K, Ramsden, D, Wu, G E
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Immunoglobulin Joining Region - genetics
Immunoglobulin kappa-Chains - genetics
Immunoglobulin Variable Region - genetics
Mice
Molecular Sequence Data
Recombination, Genetic
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Summary:The diversity of antigen-specific receptors [immunoglobulins (Ig) and T-cell receptors (Tcr)] is generated largely during the assembly of the genetic elements - named V, D, and J - that encode the variable region of both Ig and Tcr. This assembly involves a somatic, cell-type specific, site-specific rearrangement process termed V(D)J recombination. The specific sites involved in V(D)J recombination are termed recombination signal sequences (RSS). An RSS associated with an Igk-V element, for instance, and an RSS associated with an Igk-J element, together with the entire stretch of DNA separating them, are excised from the chromosome as a circular molecule, with the result that a (potentially) functional gene encoding a kappa light chain, Igk, is formed.
ISSN:0093-7711
DOI:10.1007/BF02660064