Chronic social stress produces reductions in available splenic type II corticosteroid receptor binding and plasma corticosteroid binding globulin levels

• Published in: Psychoneuroendocrinology 1996, Vol.21 (1), p.95-109
• Main Authors: Spencer, R.L., Miller, A.H., Moday, H., McEwen, B.S., Blanchard, R.J., Blanchard, D.C., Sakai, R.R.
• Format: Article
• Language: English
• Subjects: Animals; Arousal - physiology; Behavioral psychophysiology; Biological and medical sciences; Corticosteroid binding globulin; Corticosteroid receptors; Corticosterone - blood; Dominance-Subordination; Female; Fundamental and applied biological sciences. Psychology; Hormones and behavior; HPA axis; Hypothalamo-Hypophyseal System - physiopathology; Male; Pituitary-Adrenal System - physiopathology; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Rat; Rats; Receptors, Steroid - classification; Receptors, Steroid - metabolism; Social Environment; Social stress; Spleen; Spleen - innervation; Stress, Psychological - complications; Stress, Psychological - physiopathology; Transcortin - metabolism
• ISSN: 0306-4530; 1873-3360
• DOI: 10.1016/0306-4530(95)00020-8

Adult male and female rats were housed for 2 weeks in a Visible Burrow System resulting in the development of strong dominant-subordinate relationships among the male rats. Neuroendocrine measures indicated that the subordinate rats, and to a lesser extent dominant rats, experienced chronic HPA axis hyperstimulation during the 2 week experience. This paper focuses on the consequences of this chronic social stress on cytosolic type II corticosteroid receptor binding in the spleen. In the first study, rats were adrenalectomized 18 h prior to sacrifice in order to measure total cellular receptor protein levels in each animal. In spite of the severity of the social stress, there was no decrease in splenic type II corticosteroid receptor binding levels in these short-term adrenalectomized animals. In the second study, rats were left adrenal-intact. Corticosteroid receptor levels in these adrenal-intact animals reflect the level of receptors (available receptors) that were unoccupied by endogenous hormone at the time of sacrifice. Both subordinate and dominant rats had fewer available splenic type II receptors than control rats, suggesting that a greater proportion of receptors in subordinate and dominant rats were occupied and activated by endogenous hormone at the time of sacrifice than in control rats. The differences in available receptor levels were not a function of total plasma corticosterone levels at the time of sacrifice (mean corticosterone levels were the same for control and subordinate rats). Instead, the differences in available receptor levels may have been a function of plasma corticosteroid binding globulin (CBG) levels which regulate free corticosterone levels. There was a large reduction in plasma CBG levels of subordinate (−70%) and dominant (−40%) rats relative to control rats, and there was a significant correlation between plasma CBG level and available type II receptors in the spleen. These results suggest that a decrease in CBG levels as a result of chronic social stress led to greater access of free corticosterone hormone to type II receptors in the spleen than is typically present in rats under basal or acute stress conditions. This result illustrates one mechanism by which chronic stress may have a greater impact than acute stress on splenic immune function.