A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium

Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed...

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Bibliographic Details
Published in:Plasmid 1996-03, Vol.35 (2), p.71-80
Main Authors: Ridenhour, M B, Fletcher, H M, Mortensen, J E, Daneo-Moore, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Base Sequence
Cloning, Molecular
Deoxyribonuclease HindIII - metabolism
Electrophoresis, Agar Gel
Enterococcus faecium
Enterococcus faecium - drug effects
Enterococcus faecium - genetics
Humans
Minocycline - pharmacology
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids
Polymerase Chain Reaction
Tetracycline Resistance - genetics
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Description
Summary:Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed an open reading frame corresponding to an 11.8-kDa protein and containing 105 amino acid residues. There was some limited similarity between this protein and tet(M), tet(O), tet(Q), tet(S), tetB(P), and otr(A), which overlapped, but did not include, the consensus GTP-binding sequences. The low-level, Tc-resistant determinant of pKQ10, named tet(U), does not appear to correspond to any other known Tc resistance determinant.
ISSN:0147-619X
DOI:10.1006/plas.1996.0009