A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium

Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed...

Full description

Saved in:
Bibliographic Details
Published in:Plasmid 1996-03, Vol.35 (2), p.71-80
Main Authors: Ridenhour, M B, Fletcher, H M, Mortensen, J E, Daneo-Moore, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Base Sequence
Cloning, Molecular
Deoxyribonuclease HindIII - metabolism
Electrophoresis, Agar Gel
Enterococcus faecium
Enterococcus faecium - drug effects
Enterococcus faecium - genetics
Humans
Minocycline - pharmacology
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids
Polymerase Chain Reaction
Tetracycline Resistance - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 80
container_issue 2
container_start_page 71
container_title Plasmid
container_volume 35
creator Ridenhour, M B
Fletcher, H M
Mortensen, J E
Daneo-Moore, L
description Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed an open reading frame corresponding to an 11.8-kDa protein and containing 105 amino acid residues. There was some limited similarity between this protein and tet(M), tet(O), tet(Q), tet(S), tetB(P), and otr(A), which overlapped, but did not include, the consensus GTP-binding sequences. The low-level, Tc-resistant determinant of pKQ10, named tet(U), does not appear to correspond to any other known Tc resistance determinant.
doi_str_mv 10.1006/plas.1996.0009
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_78123964</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15608070</sourcerecordid><originalsourceid>FETCH-LOGICAL-g273t-340be627bdf0eabfbf6d14b812eafc0802b73e2a211382e3047f1d913d811d853</originalsourceid><addsrcrecordid>eNqFkL1PwzAUxD2ASimsbEieEEhN8bNTOxmrqnyISixUYosc-wUMidPGDlL_e1LRnemdnn53Jx0hV8BmwJi839Y6zCDP5Ywxlp-QMYNUJRLy9zNyHsLX8JUc5IiMMjUAMhuT7wX17Q_WNGLstNmb2nlMOgwuRO0jtRixa5wf9PTA3G7uptQFit60Fi1tPY2fSA_NjbN0-7IDRp2nKz_4WtMa0wdaaTSuby7IaaXrgJfHOyGbh9Xb8ilZvz4-Lxfr5IMrERORshIlV6WtGOqyKitpIS0z4KgrwzLGSyWQaw4gMo6CpaoCm4OwGYDN5mJCbv5yt1276zHEonHBYF1rj20fCjVEiVym_4Iwl0OdYgN4fQT7skFbbDvX6G5fHGcUv213cuo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15608070</pqid></control><display><type>article</type><title>A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium</title><source>ScienceDirect Journals</source><creator>Ridenhour, M B ; Fletcher, H M ; Mortensen, J E ; Daneo-Moore, L</creator><creatorcontrib>Ridenhour, M B ; Fletcher, H M ; Mortensen, J E ; Daneo-Moore, L</creatorcontrib><description>Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed an open reading frame corresponding to an 11.8-kDa protein and containing 105 amino acid residues. There was some limited similarity between this protein and tet(M), tet(O), tet(Q), tet(S), tetB(P), and otr(A), which overlapped, but did not include, the consensus GTP-binding sequences. The low-level, Tc-resistant determinant of pKQ10, named tet(U), does not appear to correspond to any other known Tc resistance determinant.</description><identifier>ISSN: 0147-619X</identifier><identifier>DOI: 10.1006/plas.1996.0009</identifier><identifier>PMID: 8700968</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Anti-Bacterial Agents - pharmacology ; Base Sequence ; Cloning, Molecular ; Deoxyribonuclease HindIII - metabolism ; Electrophoresis, Agar Gel ; Enterococcus faecium ; Enterococcus faecium - drug effects ; Enterococcus faecium - genetics ; Humans ; Minocycline - pharmacology ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Plasmids ; Polymerase Chain Reaction ; Tetracycline Resistance - genetics</subject><ispartof>Plasmid, 1996-03, Vol.35 (2), p.71-80</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8700968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ridenhour, M B</creatorcontrib><creatorcontrib>Fletcher, H M</creatorcontrib><creatorcontrib>Mortensen, J E</creatorcontrib><creatorcontrib>Daneo-Moore, L</creatorcontrib><title>A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium</title><title>Plasmid</title><addtitle>Plasmid</addtitle><description>Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed an open reading frame corresponding to an 11.8-kDa protein and containing 105 amino acid residues. There was some limited similarity between this protein and tet(M), tet(O), tet(Q), tet(S), tetB(P), and otr(A), which overlapped, but did not include, the consensus GTP-binding sequences. The low-level, Tc-resistant determinant of pKQ10, named tet(U), does not appear to correspond to any other known Tc resistance determinant.</description><subject>Amino Acid Sequence</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Deoxyribonuclease HindIII - metabolism</subject><subject>Electrophoresis, Agar Gel</subject><subject>Enterococcus faecium</subject><subject>Enterococcus faecium - drug effects</subject><subject>Enterococcus faecium - genetics</subject><subject>Humans</subject><subject>Minocycline - pharmacology</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Tetracycline Resistance - genetics</subject><issn>0147-619X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkL1PwzAUxD2ASimsbEieEEhN8bNTOxmrqnyISixUYosc-wUMidPGDlL_e1LRnemdnn53Jx0hV8BmwJi839Y6zCDP5Ywxlp-QMYNUJRLy9zNyHsLX8JUc5IiMMjUAMhuT7wX17Q_WNGLstNmb2nlMOgwuRO0jtRixa5wf9PTA3G7uptQFit60Fi1tPY2fSA_NjbN0-7IDRp2nKz_4WtMa0wdaaTSuby7IaaXrgJfHOyGbh9Xb8ilZvz4-Lxfr5IMrERORshIlV6WtGOqyKitpIS0z4KgrwzLGSyWQaw4gMo6CpaoCm4OwGYDN5mJCbv5yt1276zHEonHBYF1rj20fCjVEiVym_4Iwl0OdYgN4fQT7skFbbDvX6G5fHGcUv213cuo</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Ridenhour, M B</creator><creator>Fletcher, H M</creator><creator>Mortensen, J E</creator><creator>Daneo-Moore, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium</title><author>Ridenhour, M B ; Fletcher, H M ; Mortensen, J E ; Daneo-Moore, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g273t-340be627bdf0eabfbf6d14b812eafc0802b73e2a211382e3047f1d913d811d853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Deoxyribonuclease HindIII - metabolism</topic><topic>Electrophoresis, Agar Gel</topic><topic>Enterococcus faecium</topic><topic>Enterococcus faecium - drug effects</topic><topic>Enterococcus faecium - genetics</topic><topic>Humans</topic><topic>Minocycline - pharmacology</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Tetracycline Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ridenhour, M B</creatorcontrib><creatorcontrib>Fletcher, H M</creatorcontrib><creatorcontrib>Mortensen, J E</creatorcontrib><creatorcontrib>Daneo-Moore, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plasmid</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ridenhour, M B</au><au>Fletcher, H M</au><au>Mortensen, J E</au><au>Daneo-Moore, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium</atitle><jtitle>Plasmid</jtitle><addtitle>Plasmid</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>35</volume><issue>2</issue><spage>71</spage><epage>80</epage><pages>71-80</pages><issn>0147-619X</issn><abstract>Nine tetracycline (Tc)-resistant clinical isolates of Enterococcus faecium were screened for plasmid content using agarose gel electrophoresis. pKQ10, a 1.9-kb plasmid carrying a novel Tc resistance determinant, was isolated from one of the isolates. The nucleotide sequence of this plasmid revealed an open reading frame corresponding to an 11.8-kDa protein and containing 105 amino acid residues. There was some limited similarity between this protein and tet(M), tet(O), tet(Q), tet(S), tetB(P), and otr(A), which overlapped, but did not include, the consensus GTP-binding sequences. The low-level, Tc-resistant determinant of pKQ10, named tet(U), does not appear to correspond to any other known Tc resistance determinant.</abstract><cop>United States</cop><pmid>8700968</pmid><doi>10.1006/plas.1996.0009</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0147-619X
ispartof Plasmid, 1996-03, Vol.35 (2), p.71-80
issn 0147-619X
language eng
recordid cdi_proquest_miscellaneous_78123964
source ScienceDirect Journals
subjects Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Base Sequence
Cloning, Molecular
Deoxyribonuclease HindIII - metabolism
Electrophoresis, Agar Gel
Enterococcus faecium
Enterococcus faecium - drug effects
Enterococcus faecium - genetics
Humans
Minocycline - pharmacology
Molecular Sequence Data
Nucleic Acid Hybridization
Plasmids
Polymerase Chain Reaction
Tetracycline Resistance - genetics
title A novel tetracycline-resistant determinant, tet(U), is encoded on the plasmid pKq10 in Enterococcus faecium
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T15%3A00%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20tetracycline-resistant%20determinant,%20tet(U),%20is%20encoded%20on%20the%20plasmid%20pKq10%20in%20Enterococcus%20faecium&rft.jtitle=Plasmid&rft.au=Ridenhour,%20M%20B&rft.date=1996-03-01&rft.volume=35&rft.issue=2&rft.spage=71&rft.epage=80&rft.pages=71-80&rft.issn=0147-619X&rft_id=info:doi/10.1006/plas.1996.0009&rft_dat=%3Cproquest_pubme%3E15608070%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-g273t-340be627bdf0eabfbf6d14b812eafc0802b73e2a211382e3047f1d913d811d853%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=15608070&rft_id=info:pmid/8700968&rfr_iscdi=true