The nuclear Kluyveromyces lactis MRF1 gene encodes a mitochondrial class I peptide chain release factor that is important for cell viability

We report the isolation and characterization of the Kluyveromyces lactis MRF1 gene encoding mitochondrial peptide chain release factor mRF-1. Over-expression of the KlMRF1 gene has a strong antisuppressive effect in a Saccharomyces cerevisiae mitochondrial nonsense suppressor strain. Inactivation of...

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Bibliographic Details
Published in:Current genetics 1996-06, Vol.30 (1), p.19-28
Main Authors: Pel, H J, Rozenfeld, S, Bolotin-Fukuhara, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cell Nucleus - metabolism
Chromosome Mapping
Cloning, Molecular
Fungal Proteins - genetics
Fungal Proteins - metabolism
Genes, Bacterial
Genes, Fungal
Kluyveromyces - cytology
Kluyveromyces - genetics
Kluyveromyces - metabolism
Kluyveromyces lactis
Listeria monocytogenes - genetics
Mitochondria - metabolism
Mitochondrial Proteins
Molecular Sequence Data
Peptide Termination Factors - genetics
Peptide Termination Factors - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Sequence Homology, Amino Acid
Transcription Factors
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Summary:We report the isolation and characterization of the Kluyveromyces lactis MRF1 gene encoding mitochondrial peptide chain release factor mRF-1. Over-expression of the KlMRF1 gene has a strong antisuppressive effect in a Saccharomyces cerevisiae mitochondrial nonsense suppressor strain. Inactivation of KlMRF1 results in a dual phenotype: most cells die after about 10-13 generations, while a small number of cells exceed this limit. We propose that the lethality is related to a loss of mitochondrial genome integrity. Surviving Klmrf1 cells are able to grow slowly on the non-fermentable substrate glycerol, indicating the existence of a second mitochondrial release factor activity. Our previous comparative analysis of class I release factors is refined by the incorporation of KlmRF-1 and ten recently identified prokaryotic release factor sequences.Keywords Kluyveromyces lactis middle dot Mitochondrial release factor middle dot MRF1 middle dot Peptide chain termination
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940050095