Cloning of a cDNA for Short/Branched Chain Acyl-Coenzyme A Dehydrogenase from Rat and Characterization of Its Tissue Expression and Substrate Specificity

The acyl-CoA dehydrogenases are a family of related enzymes which catalyze the α,β-dehydrogenation of acyl-CoA esters, transferring electrons to electron-transferring flavoprotein. A cDNA for human short/branched chain acyl-CoA dehydrogenase has recently been cloned, and it has been suggested that t...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics 1996-07, Vol.331 (1), p.127-133
Main Authors: Willard, Jan, Vicanek, Caroline, Battaile, Kevin P., Van Veldhoven, Paul P., Fauq, Abdul H., Rozen, Rima, Vockley, Jerry
Format: Article
Language:English
Subjects:
Acyl-CoA Dehydrogenase
Acyl-CoA Dehydrogenases - chemistry
Acyl-CoA Dehydrogenases - genetics
Acyl-CoA Dehydrogenases - metabolism
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA, Complementary - chemistry
Gene Expression
Humans
Kidney - enzymology
Liver - enzymology
Molecular Sequence Data
Organ Specificity
Polymerase Chain Reaction
Rats
Sequence Homology
short/branched chain acyl-CoA dehydrogenase
Substrate Specificity
valproyl-CoA
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Summary:The acyl-CoA dehydrogenases are a family of related enzymes which catalyze the α,β-dehydrogenation of acyl-CoA esters, transferring electrons to electron-transferring flavoprotein. A cDNA for human short/branched chain acyl-CoA dehydrogenase has recently been cloned, and it has been suggested that this enzyme represents the human homolog for the previously reported 2-methyl branched chain acyl-CoA dehydrogenase purified from rat liver. We now report the cloning and expression of rat short/branched chain acyl-CoA dehydrogenase and characterization of its substrate specificity. The rat enzyme is more active toward longer carbon side chains than its human counterpart, while the human enzyme can utilize substrates with longer primary carbon chains. In addition, short/branched chain acyl-CoA dehydrogenase can utilize valproyl-CoA as a substrate. Northern blotting of mRNA shows ubiquitous tissue expression of both the rat and human enzyme. Further study of these enzymes will be helpful in understanding structure/function relationships in this gene family.
ISSN:0003-9861
1096-0384
DOI:10.1006/abbi.1996.0290