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Parallel measurement of oxoglutarate dehydrogenase activity and matrix free Ca2+ in fura‐2‐loaded heart mitochondria

The entrapment of the Ca2+ ‐sensitive fluorescence indicators fura‐2 or quin2 in the matrix space of isolated heart mitochondria renders possible the direct monitoring of the matrix free Ca2+ ([Ca2+]m) [(1987) Biochem. J. 248, 609–613]. In this paper the correlation between the [Ca2+]m and the in si...

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Bibliographic Details
Published in:FEBS letters 1988-02, Vol.229 (1), p.219-223
Main Authors: Lukács, Gergely L., Kapus, András, Fonyó, Attila
Format: Article
Language:English
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Summary:The entrapment of the Ca2+ ‐sensitive fluorescence indicators fura‐2 or quin2 in the matrix space of isolated heart mitochondria renders possible the direct monitoring of the matrix free Ca2+ ([Ca2+]m) [(1987) Biochem. J. 248, 609–613]. In this paper the correlation between the [Ca2+]m and the in situ activity of oxoglutarate dehydrogenase (OGDH) in fura‐2‐loaded mitochondria is shown. At the initial value of [Ca2+]m, 64 nM, which corresponded to 0.36 nmol/mg mitochondrial Ca content, the OGDH activity was 12% of the maximal. Half‐maximal and maximal activation were attained at 0.8 and 1.6 μM [Ca2+]m, respectively. The results indicate that an increase of the mitochondrial Ca content in the physiological range enhances the OGDH activity by means of elevation of [Ca2+]m.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)80831-7