Novel anthocyanins produced in petals of genetically transformed lisianthus

• Published in: Phytochemistry (Oxford) 1996-07, Vol.42 (4), p.1035-1038
• Main Author: Markham, Kenneth R.
• Format: Article
• Language: English
• Subjects: Anthocyanins - biosynthesis; Anthocyanins - chemistry; Anthocyanins - isolation & purification; Biological and medical sciences; Biotechnology; Carbohydrate Conformation; Carbohydrate Sequence; Chromatography, High Pressure Liquid; DNA, Complementary; Fundamental and applied biological sciences. Psychology; Glucosyltransferases - biosynthesis; Glucosyltransferases - metabolism; Magnetic Resonance Spectroscopy; Metabolism; Molecular Sequence Data; Molecular Structure; Plant physiology and development; Plants, Genetically Modified; Recombinant Proteins - biosynthesis; Recombinant Proteins - metabolism; Storage and secretion, pigments, phytochrome
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/0031-9422(96)00137-9

The structures of the pigments in the deep purple flowers of a lisianthus line, transformed with a UDP-glucose: flavonoid-3- O-glucosyltransferase cDNA from Antirrhinum majus, have been studied using paper chromatography, HPLC and 1H and 13C NMR spectroscopy involving the use of 1H 1H COSY and TOCSY techniques. The transgenic line is shown to have produced the new anthocyanins delphinidin-3-O-β- d-(6-O-α- l- rhamnopyranosylglucopyranoside)-5-O-β- d-[6-E ( and Z)>-p- coumaroylglucopyranoside] and delphinidin-3-O-β- d- glucopyranoside-5-O-β- d>-(6-O-E-p- coumaroylglucopyranoside) in addition to those found in the untransformed control plants.